CAJ | 학술논문

为了克隆玉米逆境胁迫应答基因，根据玉米CIPK基因EST 序列设计引物，采用RT PCR技术从玉米中克隆了1个 ZmCIPK 基因。结果表明，ZmCIPK 基因cDNA 全长1776 bp ，5′非编码区长249 bp ，3′非编码区长177 bp ，编码区长1350 bp ，编码449个氨基酸，预测分子量为50.64 kDa ，等电点为9.58。氨基酸同源性分析发现，ZmCI PK 与高粱的CI PK 同源性较高。实时定量PCR检测表明， ZmCI PK 基因的表达受脱落酸、干旱、高盐胁迫和低温诱导，说明玉米ZmCI PK基因可能参与玉米对逆境胁迫的应答。
위료극륭옥미역경협박응답기인，근거옥미CIPK기인EST 서렬설계인물，채용RT PCR기술종옥미중극륭료1개 ZmCIPK 기인。결과표명，ZmCIPK 기인cDNA 전장1776 bp ，5′비편마구장249 bp ，3′비편마구장177 bp ，편마구장1350 bp ，편마449개안기산，예측분자량위50.64 kDa ，등전점위9.58。안기산동원성분석발현，ZmCI PK 여고량적CI PK 동원성교고。실시정량PCR검측표명， ZmCI PK 기인적표체수탈락산、간한、고염협박화저온유도，설명옥미ZmCI PK기인가능삼여옥미대역경협박적응답。
In order to clone the genes responsing to abiotic stresses in maize ,the primers were de-signed based on the EST sequence of maize CIPK ,and a ZmCIPK gene was cloned from maize (Zea mays) by RT PCR method .The full length of ZmCIPK cDNA was 1 776 bp ,including a 249 bp 5′ UTR ,a 1 350 bp ORF and a 177 bp 3′ UTR .This cDNA sequence encoded a polypepide of 449 amino acid residues with a predicted molecular mass of 50 .64 kDa and a basic isoelectric point of 9 .58 .The deduced amino acid sequence had a high homology with CIPK from Sorghum bicolor .The quantitative real-time PCR result showed that the expression of ZmCIPK gene was induced by abscisic acid ,high salinity ,drought or low temperature .The expression patterns of ZmCI PK under different stresses suggested that this gene might be involved in the response to abiotic stresses .