CAJ | 학술논문

为了解鄂西北小麦主产区小麦全蚀病的变种类型，采用单孢分离法，根据病原菌培养性状、形态学特征、致病性及分子生物学特性对鄂西北4个不同县（市）小麦全蚀病病原菌进行了鉴定。结果表明：小麦茎基部叶鞘内侧成熟的全蚀病菌子囊壳黑色，光亮，圆形；子囊棒状，无色，（68．5～122）μm×（9．2～15）μm；子囊孢子线形，无色，稍弯，（73～92．5）μm×（2．8～4）μm，一般7～9个隔膜；经16srDNA测序和 PCR检测，小麦全蚀病病原菌为小麦变种（G．graminis var．tritici）和禾谷变种（G．graminis var．graminis），以小麦变种占主导地位；G．graminis var．tritici菌株致病力较强。
위료해악서북소맥주산구소맥전식병적변충류형，채용단포분리법，근거병원균배양성상、형태학특정、치병성급분자생물학특성대악서북4개불동현（시）소맥전식병병원균진행료감정。결과표명：소맥경기부협초내측성숙적전식병균자낭각흑색，광량，원형；자낭봉상，무색，（68．5～122）μm×（9．2～15）μm；자낭포자선형，무색，초만，（73～92．5）μm×（2．8～4）μm，일반7～9개격막；경16srDNA측서화 PCR검측，소맥전식병병원균위소맥변충（G．graminis var．tritici）화화곡변충（G．graminis var．graminis），이소맥변충점주도지위；G．graminis var．tritici균주치병력교강。
The pathogenic bacteria of wheat take-all disease from four counties (cities)in Northwest Hubei were identified by single-spore isolation method according to culture character, morphological characteristics,pathogenicity and molecular biological characteristics to study the variant types of wheat take-all disease in main producing area of Northwest Hubei.The results showed that the perithecium, ascus and ascospore from mature take-all pathogenic bacteria on the stem base of wheat plants was light black and roundness,colorless and rod-like(68.5~122)μm×(9.2~15)μm and colorless and linear with slightly bend and 7~9 diaphragms.The DNA sequence and PCR detection showed that the pathogenic bacteria of wheat take-all disease were G.graminis var.tritici with predominance and G.graminis var. graminis.with strong pathogenicity.