CAJ | 학술논문

水稻病毒病是水稻生产中重要的病害，目前国内流行的水稻病毒病病原多为 dsRNA病毒，每一种dsRNA病毒都有特异性基因图谱，为此，探讨了双链RNA快速小量提取法在水稻dsRNA病毒病诊断中的应用。结果表明：1）双链RNA快速小量提取法可以从0．5～0．8 g的水稻组织中快速提取 dsRNA，在3 h内完成对样品的鉴定，提取产物可以直接应用于dsRNA的检测、分类以及相关基因的克隆；2）提取效率较传统方法高，以 SRBSDV为例，传统 dsRNA提取方法中 dsRNA的产率为0．15～0．3μg/g，该方法中dsRNA的产率茎组织达到3μg/g以上，叶组织0．1～0．5μg/g；3）该方法也可以应用于介体体内的dsRNA病毒基因组核酸的提取。
수도병독병시수도생산중중요적병해，목전국내류행적수도병독병병원다위 dsRNA병독，매일충dsRNA병독도유특이성기인도보，위차，탐토료쌍련RNA쾌속소량제취법재수도dsRNA병독병진단중적응용。결과표명：1）쌍련RNA쾌속소량제취법가이종0．5～0．8 g적수도조직중쾌속제취 dsRNA，재3 h내완성대양품적감정，제취산물가이직접응용우dsRNA적검측、분류이급상관기인적극륭；2）제취효솔교전통방법고，이 SRBSDV위례，전통 dsRNA제취방법중 dsRNA적산솔위0．15～0．3μg/g，해방법중dsRNA적산솔경조직체도3μg/g이상，협조직0．1～0．5μg/g；3）해방법야가이응용우개체체내적dsRNA병독기인조핵산적제취。
Rice virus disease was an important disease in rice production,most of them were dsRNA viruses with characteristic dsRNA genome profiles.Based on that,the rapid small extraction method was explored to apply in the diagnosis of dsRNA viruses of rice disease.Results:1)by the method,0.5~0.8 g sample was required to rapidly extract dsRNA within 3 h,and the dsRNAs could be directly used to the detection and classification of dsRNA viruses and the clone of related genes;2)The extraction efficiency was higher than traditional method,taking SRBSDV as an example,the productive rate of dsRNA was 0.15~0.3μg/g,while it was more than 3μg/g from stem tissue and 0.1~0.5μg/g from leaf tissue by the rapid small extraction method;3)The method could also be used to the dsRNAs extraction from insect vectors.