CAJ | 학술논문

为了解玉米自交系耐旱性的田间鉴定与分子标记检测的一致性程度，从483份玉米自交系中筛选出田间耐旱性较好的17份，并在人为干旱胁迫下，观察鉴定了从国际玉米小麦改良中心引进的CML536、CML537、CML538和CML539的耐旱性。结果显示：CML537、CML538和 CML539的耐旱性较好；玉米dhn1基因位点的CAPS标记dhnC397检测17份自鉴耐旱系和4份 CIMMYT引进耐旱系的结果与田间耐旱性表现基本一致。用分子标记检测6份o2、o16和wx基因聚合系，其中4份不抗旱，表明有必要进一步聚合耐旱基因。结论，可利用分子标记技术初步检测玉米材料的耐旱性，此结果有利于促进分子标记辅助耐旱育种的开展。
위료해옥미자교계내한성적전간감정여분자표기검측적일치성정도，종483빈옥미자교계중사선출전간내한성교호적17빈，병재인위간한협박하，관찰감정료종국제옥미소맥개량중심인진적CML536、CML537、CML538화CML539적내한성。결과현시：CML537、CML538화 CML539적내한성교호；옥미dhn1기인위점적CAPS표기dhnC397검측17빈자감내한계화4빈 CIMMYT인진내한계적결과여전간내한성표현기본일치。용분자표기검측6빈o2、o16화wx기인취합계，기중4빈불항한，표명유필요진일보취합내한기인。결론，가이용분자표기기술초보검측옥미재료적내한성，차결과유리우촉진분자표기보조내한육충적개전。
To explore the degree of consistency of field observation and molecular marker detection of maize inbred lines for drought tolerance,2 1 maize inbred lines were observed for drought tolerance under field drought condition.The result showed that 17 lines,QCL2047,QCL2055,QCL2069,QCL2089, QCL2121,QCL2148,S611,HaiHe1,Pr(F2)(SV),Pr(F2)(V),CLQRCWQ26,CML491,QCL3053-7, CML535,CML537,CML538 and CML539,had better drought tolerance in the field.The drought tolerance of these lines was again detected by using CAPS marker of dhnC3 9 7 at dhn1 locus,and was basically consistent with the field performance, showing the molecular marker of dhnC3 9 7 could be preliminarily used for maize drought tolerance detection,and would help for promoting the development of molecular marker assisted breeding for drought tolerance.At the same time,6 lines pyramided o2、o1 6 and wx genes were tested with the marker,4 lines were not good for drought tolerance.Therefore,it is necessary to polymerize drought-tolerant gene further.