CAJ | 학술논문

目的：探讨梓醇对抗缺糖缺氧诱导的PC12细胞损伤作用。方法梓醇预处理PC12细胞，加入含连二亚硫酸钠的无糖Earle’s液制备损伤模型，检测细胞存活率，乳酸脱氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、丙二醛水平，检测细胞内游离Ca2＋浓度。结果与模型组比较，梓醇可剂量依赖性的提高细胞存活率（ P ＜0．01），减少乳酸脱氢酶漏出率（ P ＜0．05或＜0．01），提高超氧化物歧化酶、谷胱甘肽过氧化物酶活性，拮抗丙二醛水平升高，降低细胞内游离Ca2＋浓度（ P ＜0．05或＜0．01）。结论梓醇对缺糖缺氧诱导的PC12细胞损伤具有保护作用，作用机制可能与其清除自由基和抑制钙超载有关。
목적：탐토재순대항결당결양유도적PC12세포손상작용。방법재순예처리PC12세포，가입함련이아류산납적무당Earle’s액제비손상모형，검측세포존활솔，유산탈경매、초양화물기화매、곡광감태과양화물매、병이철수평，검측세포내유리Ca2＋농도。결과여모형조비교，재순가제량의뢰성적제고세포존활솔（ P ＜0．01），감소유산탈경매루출솔（ P ＜0．05혹＜0．01），제고초양화물기화매、곡광감태과양화물매활성，길항병이철수평승고，강저세포내유리Ca2＋농도（ P ＜0．05혹＜0．01）。결론재순대결당결양유도적PC12세포손상구유보호작용，작용궤제가능여기청제자유기화억제개초재유관。
Objective To investigate the protective effects of catalpol on PC 12 cells damage induced by oxygen-glucose deprivation .Methods PC12 cells were pretreated with catalpol , then exposed to sodium hyposulfite to establish cell injury model .The cell survival rates were detected by MTT assay .The activities of LDH ,SOD,GSH-Px and MDA levels were detected by colorimetry ,and free Ca2+concentration within cell was detected by fluorescence probe .Results As compared with model group ,catalpol could enhance significantly survival rate of PC12 cell in a dose-dependent manner ( P <0.01),and could reduce the efflux of LDH from damaged cell and increase the activities of SOD ,GSH-Px,however,which could decrease MDA levels and intracellular Ca2+concentration ( P <0.05 or P <0.01).Conclusion Catalpol can protect PC12 cells from oxygen glucose deprivation injury , and its action mechanism may be related with removing free radical and inhibiting calcium overload .