CAJ | 학술논문

目的：通过研究高原地区胃癌组织中THBS4基因的表达，探讨THBS4基因对高原地区胃癌生物学行为的影响及为高原地区胃预后的评估提供理论依据。方法收集高原地区无化疗且临床资料完整的胃癌手术标本60例，采用RT-PCR及S-P法，检测60例胃癌标本中THBS4蛋白的表达。结果用RT-PCR技术分析的结果显示，10例高分化胃癌组织中THBS4 mRNA有5例表达；29例低分化胃癌中24例表达，高分化癌与中、低分化癌组织中的表达有差异。中、低分化胃癌中的THBS4 mRNA水平高于高分化胃癌（ P ＜0．05）。 THBS4mRNA表达与淋巴转移有关，但与临床分期无关。免疫组化S-P法染色分析显示，THBS4蛋白在低分化29例中21例表达；中分化阳性率8例；均高于高分化（4例），THBS4在高分化癌和中、低分化癌组织中的表达差异有统计学意义（ P ＜0．05）；中、低分化胃癌中的的THBS4阳性高于高分化胃癌。 THBS4基因在高原地区胃癌组织中表达与胃癌的分化程度有关；与淋巴转移有关。结论 THBS4基因可作为判断高原地区胃癌细胞分化不良的指标，对胃癌的预后评估有重要意义。
목적：통과연구고원지구위암조직중THBS4기인적표체，탐토THBS4기인대고원지구위암생물학행위적영향급위고원지구위예후적평고제공이론의거。방법수집고원지구무화료차림상자료완정적위암수술표본60례，채용RT-PCR급S-P법，검측60례위암표본중THBS4단백적표체。결과용RT-PCR기술분석적결과현시，10례고분화위암조직중THBS4 mRNA유5례표체；29례저분화위암중24례표체，고분화암여중、저분화암조직중적표체유차이。중、저분화위암중적THBS4 mRNA수평고우고분화위암（ P ＜0．05）。 THBS4mRNA표체여림파전이유관，단여림상분기무관。면역조화S-P법염색분석현시，THBS4단백재저분화29례중21례표체；중분화양성솔8례；균고우고분화（4례），THBS4재고분화암화중、저분화암조직중적표체차이유통계학의의（ P ＜0．05）；중、저분화위암중적적THBS4양성고우고분화위암。 THBS4기인재고원지구위암조직중표체여위암적분화정도유관；여림파전이유관。결론 THBS4기인가작위판단고원지구위암세포분화불량적지표，대위암적예후평고유중요의의。
Objective To investigate the biological behaviors and clinical significance of expression of t hrombospondin4 ( THBS4 ) in gastric cancer tissues at plateau region in order to provide theoretical basis for prognosis evaluation of gastric cancer in plateau area .Mte hods The expression levels of THBS 4 protein in 60 gastric cancer specimens in plateau area were detected by RT-PCR and S-P.Results RT-PCR results showed that there were 5 cases that expressed THBS4 mRNA among 10 cases of well differentiated gastric cancers and there were 24 cases that expressed THBS 4 mRNA among 29 cases of poorly differentiated gastric cancers , there was an obvious difference in the expression of THBS 4 mRNA between well differentiated gastric cancers and moderately ,poorly differentiated gastric cancers .The expression levels of THBS4 mRNA in moderately , poorly differentiated gastric cancers were significantly higher than those in well differentiated gastric cancers ( P <0.05).The expression of THBS4mRNA was correlated to lymphatic metastasis ,however,which was not related to clinical stage.Immunohistochemistry (SP) results showed that there were 21 cases that expressed THBS4 protein among 29 cases of poorly differentiated gastric cancers and there were 8 cases that expressed THBS 4 protein in moderately differentiated gastric cancers ,which were higher than that ( 4 cases ) in well differentiated gastric cancers , and there was an significant difference in the expression of THBS4 between well differentiated gastric cancers and moderately ,poorly differentiated gastric cancers ( P <0.05).The positive rates of THBS4 expression in moderately,poorly differentiated gastric cancers were high than those in well differentiated gastric cancers .The expression of THBS4 gene in plateau area was correlated to differentiation degree and lymphatic metastasis of gastric cancers .Conclusion THBS4 gene expression can be used as the index for evaluating gastric cancer cell differentiation ,which plays an important role in prognosis evaluation of gastric cancer.