现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
1期
283-285
,共3页
乌贼膏%气相色谱法%脂肪酸组成%甲酯化%快速检测
烏賊膏%氣相色譜法%脂肪痠組成%甲酯化%快速檢測
오적고%기상색보법%지방산조성%갑지화%쾌속검측
squid offal%gas chromatography%fatty acids compositions%methyl esterification%rapid detection
研究了气相色谱法快速分析乌贼膏中脂肪酸组成的方法。通过试验比较了索式提取、超声提取和加速溶剂萃取3种方法的提取效率，同时对检测多种脂肪酸甲脂的气相色谱参数进行全面优化，建立了GC快速分析方法。结果表明，加速溶剂萃取法大大缩短了提取时间，提取出来的脂肪酸经三氟化硼-乙醚催化甲脂化，采用Agilent HP-88色谱柱分析，从乌贼膏中共检出23种脂肪酸，均达到基线分离。本方法快速、准确，可用于乌贼膏中脂肪酸组成的定性和定量分析。
研究瞭氣相色譜法快速分析烏賊膏中脂肪痠組成的方法。通過試驗比較瞭索式提取、超聲提取和加速溶劑萃取3種方法的提取效率，同時對檢測多種脂肪痠甲脂的氣相色譜參數進行全麵優化，建立瞭GC快速分析方法。結果錶明，加速溶劑萃取法大大縮短瞭提取時間，提取齣來的脂肪痠經三氟化硼-乙醚催化甲脂化，採用Agilent HP-88色譜柱分析，從烏賊膏中共檢齣23種脂肪痠，均達到基線分離。本方法快速、準確，可用于烏賊膏中脂肪痠組成的定性和定量分析。
연구료기상색보법쾌속분석오적고중지방산조성적방법。통과시험비교료색식제취、초성제취화가속용제췌취3충방법적제취효솔，동시대검측다충지방산갑지적기상색보삼수진행전면우화，건립료GC쾌속분석방법。결과표명，가속용제췌취법대대축단료제취시간，제취출래적지방산경삼불화붕-을미최화갑지화，채용Agilent HP-88색보주분석，종오적고중공검출23충지방산，균체도기선분리。본방법쾌속、준학，가용우오적고중지방산조성적정성화정량분석。
The study on the rapid analysis of fatty acids compositions in squid extract by gas chromatography (GC)was developed.The extraction rates of fat from squid extract by means of three different extraction methods,including,saxhlet extraction,ultrasonic extraction and accelerated solvent extraction were compared.Meantime by optimizing the simultaneously detection parameters of various fatty acid methyl esters by gas chromatography ,a rapid GC analysis method was established.The results showed that ,accelerated solvent extraction shorten the extraction time ,the methyl esterification of fatty acid extracted from the squid extract by using boron trifluoride etherate as catalyst ,and Agilent HP-88 column was used as analytical column , squid extract detected 23 substances ,and all reached the baseline separation.The method was rapid and accurate ,and it could be used for qualitative and quantitative analysis of fatty acids in squid extract.
