CAJ | 학술논문

目的观察不同剂量伽玛(γ)射线诱导脑膜瘤细胞凋亡和原癌基因c-myc表达变化.方法采用机械分离法获得稳定生长脑膜瘤细胞的离体培养细胞株,根据不同照射剂量分为4组,照射组分别接受中心剂量为2.00、5.00、8.22、13.33 Gy,设立对照组,剂量为0,照射后荧光显微镜观察细胞形态变化,流式细胞术检测细胞周期和凋亡率,Westem blot法检测c-myc蛋白的表达变化.结果对照组、照射1组、2组、3组及4组细胞凋亡率分别为:(3.73±0.25)％、(4.07±0.29)％、(9.81±1.66)％、(21.63±3.08)％、(8.27±1.61)％；c-myc蛋白表达分别为:0、11±0.01、0.12±0.01、0.19 ±0.02、0.31±0.08、0.09±0.01;对照组和中心剂量2 Gy组照射后细胞形态、细胞周期、凋亡率和c-myc蛋白表达差异无统计学意义(P＞0.05)；中心剂量分别为5.00 Gy和8.22 Gy组细胞出现凋亡特征,细胞周期G0/G1和G2/M期细胞数量逐渐增多,细胞凋亡率升高,c-myc蛋白表达增多,与对照组比较差异均有统计学意义(P＜0.05)；剂量为13.33 Gy组,细胞以坏死为主,S期细胞增多,c-myc蛋白表达减少.结论一定剂量的伽玛刀射线能够诱导脑膜瘤细胞进入凋亡,原癌基因c-myc在此过程中可能发挥促进脑膜瘤细胞进入凋亡过程.
목적 관찰불동제량가마(γ)사선유도뇌막류세포조망화원암기인c-myc표체변화.방법 채용궤계분리법획득은정생장뇌막류세포적리체배양세포주,근거불동조사제량분위4조,조사조분별접수중심제량위2.00、5.00、8.22、13.33 Gy,설립대조조,제량위0,조사후형광현미경관찰세포형태변화,류식세포술검측세포주기화조망솔,Westem blot법검측c-myc단백적표체변화.결과 대조조、조사1조、2조、3조급4조세포조망솔분별위:(3.73±0.25)％、(4.07±0.29)％、(9.81±1.66)％、(21.63±3.08)％、(8.27±1.61)％；c-myc단백표체분별위:0、11±0.01、0.12±0.01、0.19 ±0.02、0.31±0.08、0.09±0.01;대조조화중심제량2 Gy조조사후세포형태、세포주기、조망솔화c-myc단백표체차이무통계학의의(P＞0.05)；중심제량분별위5.00 Gy화8.22 Gy조세포출현조망특정,세포주기G0/G1화G2/M기세포수량축점증다,세포조망솔승고,c-myc단백표체증다,여대조조비교차이균유통계학의의(P＜0.05)；제량위13.33 Gy조,세포이배사위주,S기세포증다,c-myc단백표체감소.결론 일정제량적가마도사선능구유도뇌막류세포진입조망,원암기인c-myc재차과정중가능발휘촉진뇌막류세포진입조망과정.
Objective To investigate the apoptosis of meningioma cell and changes of the expression of c-myc protooncogene after gama ray radiation.Methods The isolated and cultured meningioma cells with stable growth were obtained from a patient with meningiomas by mechanical separation,and those cells were divided into 4 groups with the radiation center doses of 2.00,5.00,8.22,and 13.33 Gy respectively.There was a control group without radiation.Meningioma cell morphology was observed under fluorescent microscope.Flow cytometry was used to examine the cell cycle and apoptosis.The expression of c-myc protein was detected by using Western blotting.Results The cell apoptosis rate in contol group,and radiation 2.00,5.00,8.22 and 13.22 Gy groups was respectively (3.73 ± 0.25) ％,(4.07 ± 0.29) ％,(9.81 ± 1.66)％,(21.63 ±3.08)％ and (8.27 ± 1.61)％.Ehe expression of c-myc protein in contol group,and radiation 2.00,5.00,8.22 and 13.22 Gy groups was respectively 0.11 ± 0.01,0.12 ± 0.01,0.19 ±0.02,0.31 ±0.08 and 0.09 ±0.01.There was no significant difference in the cell morphology,cell cycle,apoptosis and the expression of c-myc protein between control group and irradiation 2.00 Gy group.Characteristics of apoptosis were found in radiation 5.00 Gy and 8.22 Gy groups,and the number of cells in G0/G1 and G2/M phases was gradually increased as well as the apoptosis rate,and the c-myc protein expression increased (P ＜ 0.05) in radiation 5.00 and 8.22 Gy groups vs.control group.In irradiation 13.33 Gy group,necrosis was found widely,the number of cells in S phase was increased and the expression of c-myc protein decreased.Conclusion The apoptosis of meningioma cells could be induced by appropriate gamma ray dose.In this process,the c-myc proto-oncogene may promote the apoptosis of meningioma cells.