CAJ | 학술논문

选用2-胺乙基膦酸双官能团小分子作为配体交换剂，采用氯仿/乙醇/水三元混合溶剂体系下的配体交换修饰方法，解决了疏水纳米晶与亲水配体的有效接触反应问题，实现了上转换纳米晶的水溶性以及表面胺基功能化修饰。通过傅里叶变转换红外光谱和热重分析证实了表面配体分子的替换。透射电子显微镜和激光粒度分析结果显示，所得水溶性纳米晶具有粒径均一，水力直径小和分散稳定的特点。样品的发射光谱结果表明，配体交换过程对纳米晶的发光无明显影响，保持了良好的发光性能。通过荧光共聚焦成像技术实现了胺基修饰上转换发光纳米晶对HeLa细胞的光学成像，证实所得纳米晶适合于潜在的生物学应用。
선용2-알을기련산쌍관능단소분자작위배체교환제，채용록방/을순/수삼원혼합용제체계하적배체교환수식방법，해결료소수납미정여친수배체적유효접촉반응문제，실현료상전환납미정적수용성이급표면알기공능화수식。통과부리협변전환홍외광보화열중분석증실료표면배체분자적체환。투사전자현미경화격광립도분석결과현시，소득수용성납미정구유립경균일，수력직경소화분산은정적특점。양품적발사광보결과표명，배체교환과정대납미정적발광무명현영향，보지료량호적발광성능。통과형광공취초성상기술실현료알기수식상전환발광납미정대HeLa세포적광학성상，증실소득납미정괄합우잠재적생물학응용。
Hydrophobic up-converting rare-earth nanoparticles( UCNPs) were transferred from organic solvent to aqueous solution via a simple surface-ligand exchange procedure. 2-Aminoethyl dihydrogen phosphate ( AEP) molecules were used to replace the original hydrophobic ligands in chloroform, ethanol and water ter-nary mixture solvent at ambient temperature. The ligand exchange process was confirmed by Fourier transform infrared spectra( FTIR) and thermogravimetric analysis( TG) . Investigations by transmission electron micros-copy(TEM), dynamic light scattering ( DLS) and photoluminescence ( PL) spectroscopy show no obvious variations in the morphologies, structure and luminescentproperties of the UCNPs during the ligand exchange process. The fluorescence confocal cell imaging of AEP modified hydrophilic UCNPs confirms that the hydro-philic UCNPs are suitable for potential biological labeling.