CAJ | 학술논문

目的：研究吡柔比星在骨肉瘤细胞MG-63的放射增敏中的作用。方法用MTT法检测不同浓度吡柔比星对对数生长期的MG-63细胞的抑制作用，确定IC10的数值，作为实验的药物浓度。用克隆形成分析法测定MG-63细胞在IC10浓度吡柔比星作用24 h后给予不同剂量（0、2、4、6、8Gy）照射以及IC10浓度紫杉醇作用不同时间（12、24、36 h）后给予一定剂量射线照射的存活分数（SF），拟合细胞生存曲线并计算放射增敏比，分析吡柔比星作用于MG-63细胞不同时间（12、24、36 h）后细胞生存率的变化。结果不同浓度吡柔比星作用24 h后，MG-63细胞抑制率逐渐增高（ P ＜0．01），其药物毒性呈浓度依赖性，IC10为0．004μg/ml。 IC10浓度吡柔比星増敏比分别1．49（D0比）、1．06（Dq比）和1．04（SF2比），MG-63细胞α/β值为2．23，IC10浓度吡柔比星作用后为0．27。 IC10浓度吡柔比星作用于MG-63细胞不同时间后再进行照射，细胞存活分数随时间延长逐渐降低（ P ＜0．01）。结论吡柔比星对MG-63细胞有放射增敏作用，在一定时间内与药物作用时间呈正相关。
목적：연구필유비성재골육류세포MG-63적방사증민중적작용。방법용MTT법검측불동농도필유비성대대수생장기적MG-63세포적억제작용，학정IC10적수치，작위실험적약물농도。용극륭형성분석법측정MG-63세포재IC10농도필유비성작용24 h후급여불동제량（0、2、4、6、8Gy）조사이급IC10농도자삼순작용불동시간（12、24、36 h）후급여일정제량사선조사적존활분수（SF），의합세포생존곡선병계산방사증민비，분석필유비성작용우MG-63세포불동시간（12、24、36 h）후세포생존솔적변화。결과불동농도필유비성작용24 h후，MG-63세포억제솔축점증고（ P ＜0．01），기약물독성정농도의뢰성，IC10위0．004μg/ml。 IC10농도필유비성증민비분별1．49（D0비）、1．06（Dq비）화1．04（SF2비），MG-63세포α/β치위2．23，IC10농도필유비성작용후위0．27。 IC10농도필유비성작용우MG-63세포불동시간후재진행조사，세포존활분수수시간연장축점강저（ P ＜0．01）。결론필유비성대MG-63세포유방사증민작용，재일정시간내여약물작용시간정정상관。
Objective To investigate the effect of pirarubicin in radiosensitization of human osteosarcoma cells (MG-63) in vitro.Methods MTT was used to measure the inhibitory effect of pirarubicin on MG -63cells at exponential growth phase to determine IC 10 as drug concentration of experiment .After MG-63 were treated with IC10 concentration pirarubicin for 24 hours,the cells were given different doses (0,2,4,6,8Gy) of irradiation as well as IC10 concentration paclitaxel for different time points (12h,24h,36h),finally the cell survival fraction (SF) was detected by clone formation analysis method,radiosensitization ratio was calculated by fitting cell survival curve ,and the changes of cell survival rate of MG-63 treated by pirarubicin in different time points (12h,24h,36 h) were analyzed.Results After 24-hour pirarubicin administration,the cell inhibition rate was gradually increased ( P <0.01),and the drug toxicity was dose -dependent,with IC10 concentration being 0.004μg/ml.According to D0,Dq and SF2 value,the sensitivity enhancement ratio ( SER) of IC10 concentration pirarubicin was 1.49,1.06 and 1.04,respectively,theα/βratio of MG-63 cells was 2.23,after treated with IC10 concentration pirarubicin,which was 0.27.After treated with IC10 concentration pirarubicin,MG-63 cells received irradiation. The cell survival fraction was gradually decreased with the time prolongation ( P <0.01).Conclusion Pirarubicin has radiosensitizing effect on MG-63 cells,and the effect is positively correlated to effect time of drug within a certain time .