CAJ | 학술논문

目的：建立酶促催化反应中底物D-半乳糖的测定方法。方法：采用高效液相色谱-蒸发光散射法（HPLC-ELSD法）测定酶促催化反应中底物D-半乳糖的含量，色谱条件：色谱柱为Diamonsil C18（2）柱（250mm×4.6mm，5μm），流动相为乙腈-水，流速为1.0ml·min-1，柱温为35℃，检测器为SEDEX-75型蒸发光散射检测器（漂移管温度为45℃，载气压力为3.0bar）。结果：D-半乳糖在0.5～10.0mg·ml-1范围内呈良好的线性关系（r=0.9995），平均回收率分别为100.3%，RSD为1.6%。结论：该方法准确、重复性好，适用于酶促催化反应中底物D-半乳糖的测定。
목적：건립매촉최화반응중저물D-반유당적측정방법。방법：채용고효액상색보-증발광산사법（HPLC-ELSD법）측정매촉최화반응중저물D-반유당적함량，색보조건：색보주위Diamonsil C18（2）주（250mm×4.6mm，5μm），류동상위을정-수，류속위1.0ml·min-1，주온위35℃，검측기위SEDEX-75형증발광산사검측기（표이관온도위45℃，재기압력위3.0bar）。결과：D-반유당재0.5～10.0mg·ml-1범위내정량호적선성관계（r=0.9995），평균회수솔분별위100.3%，RSD위1.6%。결론：해방법준학、중복성호，괄용우매촉최화반응중저물D-반유당적측정。
Objective:To establish the analysis methods for determination of D-galactose as a substrate in enzymatic reaction. Methods:HPLC-ELSD method is use to determine the content of D-galactose as a substrate in enzymatic reaction. Chromatographic conditions included Diamonsil C18(2) column(250 mm×4.6 mm,5μm),the mobile phase consisting of a mixture of acetonitrile-water. The flow rate was 1.0ml·min-1, the column temperature was 35℃and the detector was SEDEX-75 (Eva:45℃, Gas flow:3.0bar).Results:The linear range of D-galactose was 0.5~10.0mg·ml-1 (r=0.9995).The average recovery was 100.3%,RSD was 1.6%.Conclusion:The method is sensitive and with good repeatability, and it can be applicable in the detection of D-galactose as a substrate in enzymatic reation.