CAJ | 학술논문

聚乳酸(poly L-lactic acid,PLLA)及其共聚物的物理和化学性能俱佳,具有可降解性和生物相容性。以晚期内皮祖细胞(endothelial progenitor cells,EPCs)为研究对象,分别运用 CCK-8检测法、粘附能力测定实验、Boyden 小室迁移实验、Matrigel 胶成血管实验、流式细胞仪检测法、细胞衰老特异性β-半乳糖苷酶染色等方法研究聚乳酸防粘连膜对晚期EPCs功能的影响。结果显示,将晚期 EPCs 种植于聚乳酸防粘连膜上培养一定时间后,细胞的增殖、粘附、迁移、成血管功能均降低,衰老比率增高,而凋亡未见明显差异。该研究结果提示聚乳酸防粘连膜可通过抑制晚期EPCs的功能来预防感染或手术后永久性粘连的发生。
취유산(poly L-lactic acid,PLLA)급기공취물적물리화화학성능구가,구유가강해성화생물상용성。이만기내피조세포(endothelial progenitor cells,EPCs)위연구대상,분별운용 CCK-8검측법、점부능력측정실험、Boyden 소실천이실험、Matrigel 효성혈관실험、류식세포의검측법、세포쇠로특이성β-반유당감매염색등방법연구취유산방점련막대만기EPCs공능적영향。결과현시,장만기 EPCs 충식우취유산방점련막상배양일정시간후,세포적증식、점부、천이、성혈관공능균강저,쇠로비솔증고,이조망미견명현차이。해연구결과제시취유산방점련막가통과억제만기EPCs적공능래예방감염혹수술후영구성점련적발생。
To investigate the effects of the PLLA adhesion prevention membrane on the functions of late endothe-lial progenitor cells (EPCs).The 3 rd generation EPCs,namely late EPCs,was harvested and cell biological functions,such as proliferation,adhesion,migration,the ability of forming tubes,apoptosis and senescence were assayed with CCK-8,adhesion test,Boyden chamber assay,Matrigel,flow cytometry andβ-Galactosidase staining,respectively.The late EPCs implanted on the PLLA adhesions prevention membrane exhibited de-creased capabilities of proliferation,adhesion,migration and tube formation.In the mean time,PLLA increased the senescence of late EPCs but had no effect on cell apoptosis.Therefore,PLLA inhibits late EPC functions, which may be one of the mechanisms that PLLA adhesion prevention membrane prevents permanent adhesions.