CAJ | 학술논문

目的探讨全反式维A酸(ATRA)对猪胰蛋白酶(PPE)诱导肺气肿大鼠模型氧化/抗氧化因子表达的调控作用.方法选择60只SPF级健康Wistar大鼠,按数字表法随机分为对照组(N组)、模型组(E组)、棉籽油组(C组)、小剂量ATRA治疗组(L组)、中剂量ATRA治疗组(M组)和大剂量ATRA治疗组(H组)各10只作为研究对象,一次性气管内注入PPE制作大鼠肺气肿模型,然后选用小、中、大剂量ATRA及棉籽油注入大鼠腹腔内进行干预治疗.30 d后处死、解剖大鼠,测量大鼠肺体积、肺组织超氧化物歧化酶(SOD)活力和脂质过氧化物(MDA)含量.结果与N组比较,E组、C组、L组、M组、H组的肺体积明显增大(t =9.351、7.805、2.155、2.273、5.507,均P＜0.05),各组MLI、MAA的肺泡形态学比较显示:E组、C组、L组、M组、H组与N组,差异均有统计学意义(t=4.048、5.650、3.479、10.487、12.795,均P＜0.05),L组比E组明显缩小(t =5.856,P＜0.05)；E组、C组、L组、M组、H组SOD活力与N组差异均有统计学意义(t=2.711、4.398、15.767、5.672、5.415,均P＜0.05),L组SCD活力明显高于其他各组(t=15.767、13.502、12.139、7.866、7.221,均P＜0.05).E组、C组、L组、M组、H组MDA含量均明显高于N组(=7.702、8.507、3.186、7.950,均P＜0.05),L组、M组明显低于E组(t=6.397、4.044,均P＜0.05).结论采用低剂量ATRA治疗对肺泡有较好修复作用,可提高肺组织SOD活力,能够作为抗氧化作用保护剂,达到保护肺组织作用.
목적 탐토전반식유A산(ATRA)대저이단백매(PPE)유도폐기종대서모형양화/항양화인자표체적조공작용.방법 선택60지SPF급건강Wistar대서,안수자표법수궤분위대조조(N조)、모형조(E조)、면자유조(C조)、소제량ATRA치료조(L조)、중제량ATRA치료조(M조)화대제량ATRA치료조(H조)각10지작위연구대상,일차성기관내주입PPE제작대서폐기종모형,연후선용소、중、대제량ATRA급면자유주입대서복강내진행간예치료.30 d후처사、해부대서,측량대서폐체적、폐조직초양화물기화매(SOD)활력화지질과양화물(MDA)함량.결과 여N조비교,E조、C조、L조、M조、H조적폐체적명현증대(t =9.351、7.805、2.155、2.273、5.507,균P＜0.05),각조MLI、MAA적폐포형태학비교현시:E조、C조、L조、M조、H조여N조,차이균유통계학의의(t=4.048、5.650、3.479、10.487、12.795,균P＜0.05),L조비E조명현축소(t =5.856,P＜0.05)；E조、C조、L조、M조、H조SOD활력여N조차이균유통계학의의(t=2.711、4.398、15.767、5.672、5.415,균P＜0.05),L조SCD활력명현고우기타각조(t=15.767、13.502、12.139、7.866、7.221,균P＜0.05).E조、C조、L조、M조、H조MDA함량균명현고우N조(=7.702、8.507、3.186、7.950,균P＜0.05),L조、M조명현저우E조(t=6.397、4.044,균P＜0.05).결론 채용저제량ATRA치료대폐포유교호수복작용,가제고폐조직SOD활력,능구작위항양화작용보호제,체도보호폐조직작용.
Objective To observe the effect of all trans retinoic acid(ATRA) on the oxidative stress influence in emphysema model rats induced by porcine pancreatic elastase(PPE).Methods 60 rats were randomly divided into control(N),model group(E),cottonseed oil group(C),low dose of ATRA treatment group(L),medium dose of ATRA treatment group(M) and high dose of ATRA treatment group(H).The rat models of emphysema were constructed by a single intratracheal instillation of PPE and treated with low,medium and large dose of ATRA and cottonseed oil by intra-abdominal injection daily.30 days later,rats lung volume,the alveolar morphology,lung tissue SOD and MDA content were measured.Results The lung vllume of E,C,L,M,H group were significantly greater than that of N group,The alveolar morphological and lung tissue oxidative stress of E,C,L,M,H group were also significant differences compared with N group (P ＜ 0.05),and those of L group were significantly decreased compared with E group.Lung tissue SOD activity and MDA content of E,C,L,M,H group were significantly higher than N group (P ＜0.05).SOD activity of L group was higher than that of others groups (P ＜ 0.05).MDA content of L,M group were lower than E group(P ＜ 0.05).Conclusion Emphysema rat models with low dose of ATRA therapy on alveolar have better repair function,which can protect lung tissue by improving lung tissue SOD and reducing lung tissue MDA content.