CAJ | 학술논문

根据鼠伤寒沙门氏菌的特异序列,分别设计扩增引物和测序引物,建立焦磷酸测序检测鼠伤寒沙门氏菌的方法。针对鼠伤寒沙门氏菌设计特异性扩增引物,对目标片段进行PCR扩增,然后制备单链模板,并利用测序引物进行焦磷酸测序。测序结果表明,6株不同来源的鼠伤寒沙门氏菌均可以扩增出碱基序列为TACAACCGGA GTGCACATTA ATCCCGCAGC的基因片段,而30株阴性对照菌株均未得到扩增。进行BLAST比对表明,该序列与GenBank中鼠伤寒沙门氏菌的碱基序列100%匹配。焦磷酸测序法是一种快速、准确的检测方法,可用于食品中鼠伤寒沙门氏菌的快速检测。
근거서상한사문씨균적특이서렬,분별설계확증인물화측서인물,건립초린산측서검측서상한사문씨균적방법。침대서상한사문씨균설계특이성확증인물,대목표편단진행PCR확증,연후제비단련모판,병이용측서인물진행초린산측서。측서결과표명,6주불동래원적서상한사문씨균균가이확증출감기서렬위TACAACCGGA GTGCACATTA ATCCCGCAGC적기인편단,이30주음성대조균주균미득도확증。진행BLAST비대표명,해서렬여GenBank중서상한사문씨균적감기서렬100%필배。초린산측서법시일충쾌속、준학적검측방법,가용우식품중서상한사문씨균적쾌속검측。
According to the specific sequence of Salmonella typhimurium, amplification primers and sequencing primers were respectively designed to establish the pyrosequencing method in this study. Target fragments were ampli-fied with PCR by the specific primers aiming at S. typhimurium. And prepare single-stranded templates and carried out pyrosequencing with sequencing primers. The sequencing results showed that 6 strains of S. typhimurium from dif-ferent sources could all be amplified as TACAACCGGA GTGCACATTA ATCCCGCAGC gene fragments, while 30 strains of the negative control strains could not be amplified. BLAST comparison indicated that the sequences were 100% match with the base sequence of S. typhimurium in GenBank. The pyrosequencing method is a rapid, accurate detection method, it could be used for rapid detection of S. typhimurium in food.