CAJ | 학술논문

采用平板对峙法从浙江省金华市水稻田土壤中筛选获得1株拮抗水稻纹枯病菌的细菌QT-0304菌株，最大拮抗带宽达到20 mm。根据形态特征、生理生化特征、16S rRNA基因比对及进化树构树分析，鉴定QT-0304菌株为鼠乳杆菌(Lactobacillus murinus)。抗菌谱实验结果表明：QT-0304菌株对苹果腐烂病菌(Valsa mali)和杨树溃疡病病菌( Botryosphaeria dothidea)均有较好的抑制作用，拮抗带宽分别达到34.5 mm和23.0 mm。
채용평판대치법종절강성금화시수도전토양중사선획득1주길항수도문고병균적세균QT-0304균주，최대길항대관체도20 mm。근거형태특정、생리생화특정、16S rRNA기인비대급진화수구수분석，감정QT-0304균주위서유간균(Lactobacillus murinus)。항균보실험결과표명：QT-0304균주대평과부란병균(Valsa mali)화양수궤양병병균( Botryosphaeria dothidea)균유교호적억제작용，길항대관분별체도34.5 mm화23.0 mm。
A bacterial strain QT-0304 antagonizing paddy pattern blight ( Rhizoctonia solani) was obtained from paddy field soil in Jinhua, Zhejiang Province, adopting flat plate confrontation method, its maximum antagonistic band width was up to 20 mm. According to its morphological features, physiological and biochemical characteristics, 16S rRNA gene sequence comparison as well as genealogical tree construction analysis, the strain QT-0304 was identified as Lac-tobacillus murinus. The antibacterial spectrum experimental results showed that strain QT-0304 has fairly good inhibi-tion against apple decaying disease (Valsa mali) and poplar ulcer (Botryosphaeria dothidea), and the antagonistic band width could reach up to 34. 5 mm and 23. 0 mm, respectively.