CAJ | 학술논문

酿酒酵母在发酵生产乙醇的过程中存在的主要问题是前期高浓度底物葡萄糖的抑制和后期高浓度产物乙醇的抑制。功能基因组学技术的发展为从基因组水平上系统研究酿酒酵母乙醇生物合成的调控机理提供可能。本研究模拟工业发酵的条件，对酿酒酵母实验菌株BY4743为遗传背景的116个单基因缺失菌株进行了乙醇发酵试验，以发现基因和乙醇发酵的关系。结果表明乙醇对菌体得率系数高于平均值30%以上的基因缺失株有20株，其中高于50%以上基因缺失株有5株；低于平均值30%以上的基因缺失株有11株，其中低于45%以上的有5株。本研究为从整个酿酒酵母基因组水平上系统研究乙醇生物合成的调控机理建立了研究方法，提供了可行性验证。
양주효모재발효생산을순적과정중존재적주요문제시전기고농도저물포도당적억제화후기고농도산물을순적억제。공능기인조학기술적발전위종기인조수평상계통연구양주효모을순생물합성적조공궤리제공가능。본연구모의공업발효적조건，대양주효모실험균주BY4743위유전배경적116개단기인결실균주진행료을순발효시험，이발현기인화을순발효적관계。결과표명을순대균체득솔계수고우평균치30%이상적기인결실주유20주，기중고우50%이상기인결실주유5주；저우평균치30%이상적기인결실주유11주，기중저우45%이상적유5주。본연구위종정개양주효모기인조수평상계통연구을순생물합성적조공궤리건립료연구방법，제공료가행성험증。
Inhibition of high sugar concentration in early stage and inhibition of high ethanol concentration in later stage are two major problems existing in the fermentation process to produce ethanol by Saccharomyces cerevisiae. The development of functional genomics technology has made it possible to study systematically the regulation mechanism of ethanol biosynthesis at genomic level. Industrial fermentation conditions was simulated in this study to carry out etha-nol fermentation experiments to discover relationship between genes and ethanol fermentation, taking 116 yeast single-gene deletion strains of BY4743 genetic background as ethanol experimental strains. The results showed that there were 20 gene-deletion strains with gain rate coefficient of ethanol to thalamus higher than 30% of the average, among them 5 gene-deletion strains were 50% higher; while those lower than 30% of the average were 11 strains, among them there were 5 strains being lower than 45%. This study has established research methods for regulation mecha-nisms at genomic level of S. cerevisae systematically to study ethanol biosynthesis as a whole, and provided feasibility tests and verifications.