CAJ | 학술논문

在单因素试验的基础上，通过正交试验对超声波辅助提取金丝草总黄酮的工艺条件进行优化.并采用水杨酸法测定其清除羟自由基能力、邻苯三酚自氧化法测定其清除超氧自由基能力和普鲁士蓝法测定还原能力．结果表明，提取金丝草总黄酮的最佳工艺条件为：乙醇浓度50%，料液比1∶35，超声波功率70 W，超声时间35 min，超声温度47℃，总黄酮得率为3.08%.金丝草黄酮提取物对羟自由基和超氧自由基呈现出清除作用，还原能力则随着浓度的增加而增强.
재단인소시험적기출상，통과정교시험대초성파보조제취금사초총황동적공예조건진행우화.병채용수양산법측정기청제간자유기능력、린분삼분자양화법측정기청제초양자유기능력화보로사람법측정환원능력．결과표명，제취금사초총황동적최가공예조건위：을순농도50%，료액비1∶35，초성파공솔70 W，초성시간35 min，초성온도47℃，총황동득솔위3.08%.금사초황동제취물대간자유기화초양자유기정현출청제작용，환원능력칙수착농도적증가이증강.
On the base of the single factor experiments,the optimal processing conditions of ultra-sound-assisted for the extraction of total flavonoids from Pogonatherum crinitum were obtained by orthogonal experiments and the antioxidation of total flavonoids by the elimination capacity on hydroxyl radical with sali-cylic acid method,superoxide anion radical with pyrogallol oxidation method and the deoxidize activity with prussian blue method were studied.The results indicated that the optimum conditions were immersed in 47℃50%ethanol with solid-liquid ratio 1∶35 for 35 min by ultrasonic power 70 W. Its yield was 3.080%.The ex-traction had certain elimination effects on hydroxyl radical and superoxide anion radical.