世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
9期
2047-2051
,共5页
陆一竹%黄照环%张茜薇%宋卓敏%王娜
陸一竹%黃照環%張茜薇%宋卓敏%王娜
륙일죽%황조배%장천미%송탁민%왕나
子宫平滑肌瘤癥益气消%法%微血管密度%血管内皮生长因子%血管内皮生长因子受体
子宮平滑肌瘤癥益氣消%法%微血管密度%血管內皮生長因子%血管內皮生長因子受體
자궁평활기류징익기소%법%미혈관밀도%혈관내피생장인자%혈관내피생장인자수체
Uterine fibroids%Y i-Qi Xiao-Zheng method%microvessel density%vascular endothelial growth factor%vascular endothelial growth factor receptor
癥目的：观察益气消法对雌激素诱导子宫肌瘤模型豚鼠血管内皮生长因子及其受体蛋白表达的影响，探讨该法方药抑制血管生成治疗子宫肌瘤的作用机制。方法：通过去势+皮下注射苯甲酸雌二醇建癥立子宫肌瘤模型，并给予不同剂量益气消法中药进行治疗。采用免疫组化法检测子宫组织微血管密度（MVD）、血管内皮生长因子（VEGF）蛋白表达水平；采用RT-PCR检测子宫组织VEGFR1 mRNA、VEGFR2 mRNA表达。并对以上指标进行统计学分析。结果：与正常对照组比较，模型组豚鼠子宫肌组织MVD、VEGF、VEGFR表达显著升高（P<0.01）；与模型组比较，中药各剂量组均有不同程度的降低，其中以高剂量癥组最为明显，说明益气消法高剂量中药组可以显著抑制MVD、VEGF及其受体的蛋白表达。结论：益气消癥法通过抑制血管生成进而抗肌瘤细胞增殖，这可能是该法治疗子宫肌瘤的作用机制之一。
癥目的：觀察益氣消法對雌激素誘導子宮肌瘤模型豚鼠血管內皮生長因子及其受體蛋白錶達的影響，探討該法方藥抑製血管生成治療子宮肌瘤的作用機製。方法：通過去勢+皮下註射苯甲痠雌二醇建癥立子宮肌瘤模型，併給予不同劑量益氣消法中藥進行治療。採用免疫組化法檢測子宮組織微血管密度（MVD）、血管內皮生長因子（VEGF）蛋白錶達水平；採用RT-PCR檢測子宮組織VEGFR1 mRNA、VEGFR2 mRNA錶達。併對以上指標進行統計學分析。結果：與正常對照組比較，模型組豚鼠子宮肌組織MVD、VEGF、VEGFR錶達顯著升高（P<0.01）；與模型組比較，中藥各劑量組均有不同程度的降低，其中以高劑量癥組最為明顯，說明益氣消法高劑量中藥組可以顯著抑製MVD、VEGF及其受體的蛋白錶達。結論：益氣消癥法通過抑製血管生成進而抗肌瘤細胞增殖，這可能是該法治療子宮肌瘤的作用機製之一。
징목적：관찰익기소법대자격소유도자궁기류모형돈서혈관내피생장인자급기수체단백표체적영향，탐토해법방약억제혈관생성치료자궁기류적작용궤제。방법：통과거세+피하주사분갑산자이순건징립자궁기류모형，병급여불동제량익기소법중약진행치료。채용면역조화법검측자궁조직미혈관밀도（MVD）、혈관내피생장인자（VEGF）단백표체수평；채용RT-PCR검측자궁조직VEGFR1 mRNA、VEGFR2 mRNA표체。병대이상지표진행통계학분석。결과：여정상대조조비교，모형조돈서자궁기조직MVD、VEGF、VEGFR표체현저승고（P<0.01）；여모형조비교，중약각제량조균유불동정도적강저，기중이고제량징조최위명현，설명익기소법고제량중약조가이현저억제MVD、VEGF급기수체적단백표체。결론：익기소징법통과억제혈관생성진이항기류세포증식，저가능시해법치료자궁기류적작용궤제지일。
This study was aimed to observe the influence on angiogenesis factor and expression of its receptor protein on estrogen-induced uterine fibroids rat models with Y i-Qi Xiao-Zheng (YQXZ) method, in order to explore the mechanism of this method in the inhibition of angiogenesis in the treatment of uterine fibroids. Castration and estro-gen-induced method were used in the establishment of guinea pig uterine fibroids rat model. And then, different doses of Chinese medicine of YQXZ method were given. The immunohistochemical method was used in the detection of microvessel density (MVD) and vascular endothelial growth factor (VEGF) protein expression of the uterine tissues. The RT-PCR method was used to detect the expression of VEGFR1 mRNA and VEGFR2 mRNA. Then, statistical analysis was given on indexes mentioned above. The results showed that compared with the normal control group, ex-pression of MVD, VEGF and VEGFR of uterine muscle tissues in the model group was significantly higher (P <0.01). Compared with the model group, Chinese medicine group of different doses showed different reducing. Among them, the high-dose group was the most obvious one. It showed that the high-dose Chinese medicine of YQXZ group was able to significantly inhibit the expression of MVD, VEGF and theirs receptors. It was concluded that YQXZ method can antagonize proliferation of the leiomyoma cell by inhibiting angiogenesis. Therefore, it may be one of the mechanisms of this method in the treatment of uterine fibroids.
