CAJ | 학술논문

目的：建立注射用银杏二萜内酯中甘露醇和葡甲胺含量测定方法。方法：采用HPLC-ELSD法，以乙腈-50 mmol·mL-1乙酸铵为流动相，色谱柱Waters XBridge Amide（4.6 mm×150 mm，3.5μm）；梯度洗脱，流速1.0 mL·min-1；柱温30℃；漂移管温度90℃，氮气流速2.0 L·min-1。结果：甘露醇在1.9665~19.665μg范围内呈良好线性关系，平均回收率为100.57%（RSD=0.92%，n=9）；葡甲胺在0.4838~4.638μg范围内呈良好线性关系，平均回收率为100.67%（RSD=0.72%，n=9）。结论：该方法简便准确，重复性好，可作为测定注射用银杏二萜内酯中甘露醇和葡甲胺的含量测定方法。
목적：건립주사용은행이첩내지중감로순화포갑알함량측정방법。방법：채용HPLC-ELSD법，이을정-50 mmol·mL-1을산안위류동상，색보주Waters XBridge Amide（4.6 mm×150 mm，3.5μm）；제도세탈，류속1.0 mL·min-1；주온30℃；표이관온도90℃，담기류속2.0 L·min-1。결과：감로순재1.9665~19.665μg범위내정량호선성관계，평균회수솔위100.57%（RSD=0.92%，n=9）；포갑알재0.4838~4.638μg범위내정량호선성관계，평균회수솔위100.67%（RSD=0.72%，n=9）。결론：해방법간편준학，중복성호，가작위측정주사용은행이첩내지중감로순화포갑알적함량측정방법。
This study was aimed to develop the method for determination of mannitol and meglumine in diterpene lactones of Ginkgo Injection. The HPLC-ELSD was used to determine mannitol and meglumine in diterpene lactones of Ginkgo Injection. The analysis was carried out on Waters XBridge Amide (4.6 mm í 150 mm, 3.5 μm) column. The mobile phase was the mixture of acetonitrile, and 50 mmol·mL-1 ammonium acetate with a linear gradient elu-tion at a flow rate of 1.0 mL·min-1. The column temperature wad maintained at 30℃. The operating conditions of the ELSD were a nebulizer nitrogen with the flow rate of 2.0 L·min-1 and an evaporator tube at the temperature of 90℃. The results showed that mannitol had a good linear relationship in a range of 1.9665 μg - 19.665 μg. The average recovery rate was 100.57% (RSD = 0.92%, n = 9). The meglumine showed a good linear relationship in a range of 0.4838 μg - 4.638 μg. The average recovery rate was 100.67% (RSD = 0.72%, n = 9). It was concluded that this method was simple and accurate with good reproducibility. It met with the requirement of the determination of the contents of mannitol and meglumine in diterpene lactones of Ginkgo Injection.