CAJ | 학술논문

目的：选取晶体生长速率和（COM和聚集体量）/总晶体量比例为评价指标，优化体外模拟草酸钙结石模型条件。方法：采用琼脂凝胶双向扩散法，选取琼脂凝胶浓度、培养温度、培养时间、氯化钙和草酸钠配比4个因素进行正交试验，通过光学显微镜定时观察对草酸钙晶体晶型的生成，优选出最佳体外草酸钙结晶形成的条件。结果：随着培养时间的延长，不同浓度琼脂凝胶均呈现一定比例的一水草酸钙（COM）、二水草酸钙（COD）、三水草酸钙（COT）及聚集体，其中以1%琼脂浓度下的聚集速率最快且易形成COM；培养温度在37℃时最利于COM的成核、生长以及聚集；0.1/0.2 mol·L-1氯化钙/草酸钠，在37℃下培养3天，生成的COM及聚集体比例最大，达总晶体量90%以上。结论：pH7.4、1%琼脂及0.1/0.2 mol·L-1氯化钙/草酸钠的琼脂凝胶研究系统在37℃下培养3天，为复制以COM为主的体外草酸钙结石模型最佳条件。
목적：선취정체생장속솔화（COM화취집체량）/총정체량비례위평개지표，우화체외모의초산개결석모형조건。방법：채용경지응효쌍향확산법，선취경지응효농도、배양온도、배양시간、록화개화초산납배비4개인소진행정교시험，통과광학현미경정시관찰대초산개정체정형적생성，우선출최가체외초산개결정형성적조건。결과：수착배양시간적연장，불동농도경지응효균정현일정비례적일수초산개（COM）、이수초산개（COD）、삼수초산개（COT）급취집체，기중이1%경지농도하적취집속솔최쾌차역형성COM；배양온도재37℃시최리우COM적성핵、생장이급취집；0.1/0.2 mol·L-1록화개/초산납，재37℃하배양3천，생성적COM급취집체비례최대，체총정체량90%이상。결론：pH7.4、1%경지급0.1/0.2 mol·L-1록화개/초산납적경지응효연구계통재37℃하배양3천，위복제이COM위주적체외초산개결석모형최가조건。
This study was aimed to optimize the influential factors on simulation calcium oxalate calculus model in vitro with the targets of the CaOxa crystal growth rate and the ratio of CaOxa crystals. The model of calcium oxalate stone agar gel system was established in order to evaluate four influential factors which include the concentration of agar gel, incubation temperature, incubation time, calcium chloride / sodium oxalate for the orthogonal test. The for-mation of calcium oxalate stone was observed under the microscope. Then, the formation conditions of calcium ox-alate stone in vitro were optimized. The results showed that as the extension of incubation time, different concentra-tions of agar gel were rendered in a certain percentage of COM, COD, COT as well as aggregates. Among them, the gathered rate was the quickest and easy to form COM under 1% agar concentration. When the cultivation con-dition was 37℃, it was the most suitable condition for COM nucleation, growth, and gather. With concentration of calcium chloride / sodium oxalate as 0.1/0.2 mol/L, incubating in 37℃ for 3 days, COM and aggregate accounted for more 90% of all crystals. It was concluded that the optimal conditions for the model of calcium oxalate stone formation in vitro were incubated in 37℃ for 3 days with 1% agar gel, calcium chloride / sodium oxalate as 0.1/0.2 mol/L at pH 7.4.