CAJ | 학술논문

目的：探讨金银花种质资源的遗传多样性，为其育种提供参考。方法：对采自金银花主产区的18个农家品种及野生忍冬和近缘种灰毡毛忍冬共计36个样品进行 ISSR-PCR 分析，采用NTSYS-pc软件计算金银花不同农家品种及近缘种间的Jaccard遗传相似系数，按非加权配对算术平均法（UPGMA）建立所研究类群的系统聚类图。结果：12条引物共扩增出129个条带，其中多态带为114条，占总扩增条带数的88.37%，金银花种质资源具有较丰富的遗传多样性。从聚类分析图可以看出，金银花不同样本首先聚在一起，表明是一自然类群；野生忍冬与栽培品种具有明显的遗传差异；主产区传统品系毛花系列遗传相对较稳定，而鸡爪花系列品种繁杂，遗传变异大；新品种九丰一号已独立成一稳定品种。结论：ISSR标记可以为金银花的种质资源鉴定、遗传关系分析及栽培育种提供分子生物学依据。
목적：탐토금은화충질자원적유전다양성，위기육충제공삼고。방법：대채자금은화주산구적18개농가품충급야생인동화근연충회전모인동공계36개양품진행 ISSR-PCR 분석，채용NTSYS-pc연건계산금은화불동농가품충급근연충간적Jaccard유전상사계수，안비가권배대산술평균법（UPGMA）건립소연구류군적계통취류도。결과：12조인물공확증출129개조대，기중다태대위114조，점총확증조대수적88.37%，금은화충질자원구유교봉부적유전다양성。종취류분석도가이간출，금은화불동양본수선취재일기，표명시일자연류군；야생인동여재배품충구유명현적유전차이；주산구전통품계모화계렬유전상대교은정，이계조화계렬품충번잡，유전변이대；신품충구봉일호이독립성일은정품충。결론：ISSR표기가이위금은화적충질자원감정、유전관계분석급재배육충제공분자생물학의거。
This paper was aimed to study the genetic diversity and genetic relationship of germplasm resource of Lonicerae Japonicae Flos in order to provide references for its breeding. A total of 36 samples from 18 farm varieties and wild species, as well as related species of Lonicera japonica Thunb. from the main production areas were studied by ISSR-PCR markers. The Jaccard coefficient was worked out by NTSYS-pc software. And a cluster dendrogram of different samples was established based on unweighted pair-group method with arithmetic mean (UPGMA). The re-sults showed that 12 ISSR primers generated 129 loci of which 114 loci were polymorphic. The average percentage of polymorphic bands (PPB) is 88.37%. In the cluster dendrogram, different samples of Lonicera japonica are in the same group, which showed that it is a natural species; the wild sample is separated from the cultivated samples; the traditional type-Maohua is relative stable, but the type of Jizhuahua includes complicated varieties, and it has obvi-ous genetic variation; the new variety Jiufeng 1 has already distinct into one stable type. It was concluded that the ISSR method was suitable for germplasm identification, genetic diversity analysis of Lonicerae Japonicae Flos, thus providing a theoretical foundation for its cultivation and breeding.