CAJ | 학술논문

目的：通过优化的均匀设计方法，以脂质代谢、血小板功能、血管活性物质和炎症因子等药理学指标，对理气活血中药配伍（简称气血并治方）水溶性提取物进行配伍配比，寻找和辨识其防治动脉粥样硬化的有效组分及最佳配比。方法：将气血并治方水溶性提取物通过大孔树脂按照不同极性分为6组分，运用优化的均匀设计方法进行不同比例的配伍，观察各配伍组分对高脂血症模型大鼠的血脂水平、血小板聚集性、炎症因子和血管内皮因子的影响，比较其作用强度和作用环节的差异。结果：通过优化均匀设计的药理实验和统计分析，与模型组比较，气血并治方水溶性提取物的D组分（主要为芍药苷，约占49.12%）、E组分（主要为总黄酮，约占30.0%）配伍具有显著降低血清胆固醇和甘油三酯水平的作用（P<0.01），同时对血浆内皮素（ET）和血小板最大聚集率有显著抑制作用（P<0.01）。方中F组分（主要为总酸，约占32.7%）具有抑制血清中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）水平的作用（P<0.01）。结论：气血并治方中药水溶性提取物不同配伍可作用于机体的不同靶点或环节，其全方提取物的有效组分主要是芍药苷、黄酮类、总酸，最佳比例约为1、1、1。
목적：통과우화적균균설계방법，이지질대사、혈소판공능、혈관활성물질화염증인자등약이학지표，대리기활혈중약배오（간칭기혈병치방）수용성제취물진행배오배비，심조화변식기방치동맥죽양경화적유효조분급최가배비。방법：장기혈병치방수용성제취물통과대공수지안조불동겁성분위6조분，운용우화적균균설계방법진행불동비례적배오，관찰각배오조분대고지혈증모형대서적혈지수평、혈소판취집성、염증인자화혈관내피인자적영향，비교기작용강도화작용배절적차이。결과：통과우화균균설계적약리실험화통계분석，여모형조비교，기혈병치방수용성제취물적D조분（주요위작약감，약점49.12%）、E조분（주요위총황동，약점30.0%）배오구유현저강저혈청담고순화감유삼지수평적작용（P<0.01），동시대혈장내피소（ET）화혈소판최대취집솔유현저억제작용（P<0.01）。방중F조분（주요위총산，약점32.7%）구유억제혈청중백세포개소-6（IL-6）、백세포개소-8（IL-8）수평적작용（P<0.01）。결론：기혈병치방중약수용성제취물불동배오가작용우궤체적불동파점혹배절，기전방제취물적유효조분주요시작약감、황동류、총산，최가비례약위1、1、1。
This experiment was designed to search and identify the active principle as well as the optimal proportion of water-soluble extractives of traditional Chinese medicine (water-soluble extractives) Liqi Huoxue medicinals com-patibility (Qixue Bingzhi Fang-CWQB) in the prevention and treatment of atherosclerosis (As) by optimal uniform design method. The water-soluble extractives of CWQB were divided into 6 sections (A, B, C, D, E, F) through macroporous resin. The effect intensity and step of every component were compared through its effect on blood fat level, platelet aggregation, inflammatory factors, vascular endothelial growth factor (VEGF) and so on among hyper-lipoidemia rat models. The pharmacological experimental results and statistical analysis showed that CWQB water-soluble extractives of component D (mainly is paeoniflorin, accounted for 49.12%), component E (mainly is total flavonoids, accounted for 30.0%) compatibility had better effects on decreasing blood fat and triglyceride (TG). Com-pared with the model group, there was significant difference (P < 0.01). It also had inhibiting effect on endothelin (ET) and maximum platelet aggregation rate (P < 0.01). The component F (mainly is total acids, accounted for 32.7%) had inhibiting effect on serum IL-6 and IL-8 (P< 0.01). It was concluded that different compatibility of wa-ter-soluble extractives of CWQB can be applied to different targets or steps of the body. The active principle extrac-tives include main component of paeoniflorin, flavonoids and total acids. The best proportion is about 1:1:1.