CAJ | 학술논문

目的：对麻花秦艽和粗茎秦艽叶绿体基因组进行提取研究，为进一步研究秦艽叶绿体的基因组序列特征、遗传多样性以及资源保护研究奠定基础。方法：本文以麻花秦艽、粗茎秦艽为对象，采用改良的蔗糖密度梯度离心方法进行提取，优化纯化方法，分离得到秦艽叶绿体后测定cpDNA 浓度，检测其OD值并扩增ITS2序列验证其纯度。结果：获得高质量、高纯度的 cpDNA (0.1~0.4滋g/10 g)，扩增ITS2序列验证其无核污染，满足后续测序要求。结论：cpDNA 的纯度和质量对叶绿体基因组测序的正确率和可信度具有重要的影响。本文获得了高纯度、高质量的秦艽cpDNA，为秦艽叶绿体基因组全序列的获得提供保障。
목적：대마화진구화조경진구협록체기인조진행제취연구，위진일보연구진구협록체적기인조서렬특정、유전다양성이급자원보호연구전정기출。방법：본문이마화진구、조경진구위대상，채용개량적자당밀도제도리심방법진행제취，우화순화방법，분리득도진구협록체후측정cpDNA 농도，검측기OD치병확증ITS2서렬험증기순도。결과：획득고질량、고순도적 cpDNA (0.1~0.4자g/10 g)，확증ITS2서렬험증기무핵오염，만족후속측서요구。결론：cpDNA 적순도화질량대협록체기인조측서적정학솔화가신도구유중요적영향。본문획득료고순도、고질량적진구cpDNA，위진구협록체기인조전서렬적획득제공보장。
This study was aimed to show the extraction research of cpDNA in Gentiana straminea Maxim. and G. crassicaulis Duthie ex Burk., which will increase the knowledge about the chloroplast genome sequence characteris-tics, understand its genetic diversity and improve the efficiency of breeding schemes. G. straminea Maxim. and G. crassicaulis Duthie ex Burk. were taken as study objects. The improved sucrose-gradient centrifugation and purifica-tion methods were used to obtain the cpDNA, measure the concentration, detect the OD value, and amplify the ITS2 sequence to verify the nucleus pollution. The results showed that the high quality and purity cpDNA (0.1 - 0.4 μg/10 g) was demonstrated without other pollution after ITS2 sequence amplification, which met to the subsequent effi-cient sequencing of chloroplast genomes. The purity and mass have an important influence on the accuracy and cred-ibility of the cp genome sequencing. It was concluded that this study improved the sucrose-gradient centrifugation and purification with great effect of the purity and mass of cpDNA, and guaranteed the obtaining of complete cpDNA of Gentiana.