国际泌尿系统杂志
國際泌尿繫統雜誌
국제비뇨계통잡지
INTERNATIONAL JOURNAL OF UROLOGY AND NEPHROLOGY
2013年
4期
439-441
,共3页
隐睾%疾病模型,动物%降钙素基因相关肽%小鼠
隱睪%疾病模型,動物%降鈣素基因相關肽%小鼠
은고%질병모형,동물%강개소기인상관태%소서
Cryptorchidism%Disease Models,Animal%Calcitonin Gene-Related Peptide%Mice
目的 通过氟他胺(Flutamide,FLU)诱导小鼠隐睾动物模型的建立,探索其睾丸组织中降钙素基因相关肽(Calcitonin gene-related peptide,CGRP) mRNA表达的变化.方法 将妊娠BALB/c小鼠随机分成A、B、C、D、E5组；在母鼠孕第12～21d持续10d给予A、B、C、D、E5组氟他胺灌胃,剂量依次为0mg/kg、150mg/kg、300 mg/kg、500 mg/kg、700 mg/kg,A组为空白对照组；在出生后第8周时运用Real-Time PCR技术检测子代小鼠睾丸组织中CGRP mRNA的表达情况及单侧隐睾小鼠中患侧与健侧睾丸CGRP mRNA的表达情况.结果 A组小鼠无隐睾,B、C组有单侧隐睾；D、E组有双侧隐睾的发生.与对照组相比,随着给药剂量的增大,CGRP mRNA的表达强度明显低于对照组(P＜0.01).单侧隐睾小鼠中患侧和对侧睾丸的CGRPmRNA均减少(P＜0.05).结论 剂量为150mg/kg与300 mg/kg的氟他胺能诱导产生单侧隐睾模型,剂量为500与700 mg/kg的氟他胺能诱导产生双侧隐睾模型；单侧隐睾小鼠中双侧睾丸均有损害；CGRP是隐睾产生过程中一个重要的影响因素.
目的 通過氟他胺(Flutamide,FLU)誘導小鼠隱睪動物模型的建立,探索其睪汍組織中降鈣素基因相關肽(Calcitonin gene-related peptide,CGRP) mRNA錶達的變化.方法 將妊娠BALB/c小鼠隨機分成A、B、C、D、E5組；在母鼠孕第12～21d持續10d給予A、B、C、D、E5組氟他胺灌胃,劑量依次為0mg/kg、150mg/kg、300 mg/kg、500 mg/kg、700 mg/kg,A組為空白對照組；在齣生後第8週時運用Real-Time PCR技術檢測子代小鼠睪汍組織中CGRP mRNA的錶達情況及單側隱睪小鼠中患側與健側睪汍CGRP mRNA的錶達情況.結果 A組小鼠無隱睪,B、C組有單側隱睪；D、E組有雙側隱睪的髮生.與對照組相比,隨著給藥劑量的增大,CGRP mRNA的錶達彊度明顯低于對照組(P＜0.01).單側隱睪小鼠中患側和對側睪汍的CGRPmRNA均減少(P＜0.05).結論 劑量為150mg/kg與300 mg/kg的氟他胺能誘導產生單側隱睪模型,劑量為500與700 mg/kg的氟他胺能誘導產生雙側隱睪模型；單側隱睪小鼠中雙側睪汍均有損害；CGRP是隱睪產生過程中一箇重要的影響因素.
목적 통과불타알(Flutamide,FLU)유도소서은고동물모형적건립,탐색기고환조직중강개소기인상관태(Calcitonin gene-related peptide,CGRP) mRNA표체적변화.방법 장임신BALB/c소서수궤분성A、B、C、D、E5조；재모서잉제12～21d지속10d급여A、B、C、D、E5조불타알관위,제량의차위0mg/kg、150mg/kg、300 mg/kg、500 mg/kg、700 mg/kg,A조위공백대조조；재출생후제8주시운용Real-Time PCR기술검측자대소서고환조직중CGRP mRNA적표체정황급단측은고소서중환측여건측고환CGRP mRNA적표체정황.결과 A조소서무은고,B、C조유단측은고；D、E조유쌍측은고적발생.여대조조상비,수착급약제량적증대,CGRP mRNA적표체강도명현저우대조조(P＜0.01).단측은고소서중환측화대측고환적CGRPmRNA균감소(P＜0.05).결론 제량위150mg/kg여300 mg/kg적불타알능유도산생단측은고모형,제량위500여700 mg/kg적불타알능유도산생쌍측은고모형；단측은고소서중쌍측고환균유손해；CGRP시은고산생과정중일개중요적영향인소.
Objectives To establish a mice model of cryptorchidism induced by flutamide,and further study the change expression of testicular Calcitonin gene-related peptide message-RNA.Methods The pregnancy BALB/c mice were divided into five groups A,B,C,D and E randomly; Flutamide was consecutive gavage administrated at 0mg/kg、50mg/kg、300mg/kg、500mg/kg、700mg/kg from gestation day 12 to 21.Group A was control group; And then detected expression of testicular CGRP mRNA by Real-time PCR on postnatal eight weeks.And compare the expression of CGRP mRNA between downthrow side and health side in Unilateral cryptorchidism.Results No cryptorchidism in group A,the unilateral cryptorchidism was seen in group B and C,and the bilateral cryptorchidism was seen in group D and E;With the increase dose of the flutamide,the expression of CGRP mRNA gradually reduced (P ＜ 0.01) ;And the same with the unilateral cryptorchidism(P ＜ 0.05).Conclusions The unilateral cryptorchidism model can be induced by flutamide at the does of 300 mg/kg and 150 mg/kg,the bilateral cryptorchism model can be induced at the does of 500 mg/kg and 700 mg/kg ;both sides of testis were damaged in unilateral cryptorchidism;CGRP is an important influence factors in the cryptorchidism produce process.
