中华危重病急救医学
中華危重病急救醫學
중화위중병급구의학
Chinese Critical Care Medicine
2013年
4期
233-237
,共5页
刘景全%石斌%马国光%娄晓丽%刘鸿翔%梁冬雨%万晟霞
劉景全%石斌%馬國光%婁曉麗%劉鴻翔%樑鼕雨%萬晟霞
류경전%석빈%마국광%루효려%류홍상%량동우%만성하
脓毒症%Ghrelin%短肽载体1%盲肠结扎穿孔术
膿毒癥%Ghrelin%短肽載體1%盲腸結扎穿孔術
농독증%Ghrelin%단태재체1%맹장결찰천공술
Sepsis%Ghrelin%PepT1%Cecal ligation and puncture
目的 探讨脑肠肽Ghrelin对脓毒症大鼠小肠上皮短肽载体1(PepT1)表达及功能的影响.方法 雄性SD大鼠80只,按随机数字表法分为正常组、假手术组、脓毒症组和Ghrelin干预组4组,每组20只.采用盲肠结扎穿孔术(GLP)制备脓毒症大鼠模型;制模后即静脉给予Ghrelin干预.各组术后20 h随机取10只大鼠,进行小肠黏膜病理观察,实时定量聚合酶链反应(PCR)和蛋白质免疫印迹试验分别检测PepT1的mRNA和蛋白表达,同时检测小肠上皮PepT1对底物Gly-Sar的摄取浓度;另10只大鼠记录7d生存率.结果 与正常组和假手术组相比,脓毒症组小肠黏膜受损明显,PepT1 mRNA表达(21.43±1.37比23.29±1.24、23.23±1.48)、蛋白表达(7832.55±699.74比13 584.74±360.66、13 152.51±605.72)以及Gly-Sar浓度(60 min:3.23±0.30比6.67±0.51、6.52±0.47)均明显降低(均P<0.05);与脓毒症组比较,Ghrelin干预组大鼠小肠黏膜损伤较轻,生存率(40%比20%)、PepT1 mRNA表达(22.73±1.16比21.43±1.37)、蛋白表达(10 506.26±850.59比7832.55±699.74)及Gly-Sar浓度(60 min:4.83±0.32比3.23±0.30)均显著升高(均P<0.05).正常组与假手术组各指标比较无显著差异.结论 脓毒症大鼠小肠上皮PepT1mRNA及蛋白表达明显下降,机体在基因及蛋白水平下调了小肠上皮PepT1生物学功能;Ghrelin干预对脓毒症小肠上皮PepT1 mRNA、蛋白表达以及摄取能力均有上调作用.
目的 探討腦腸肽Ghrelin對膿毒癥大鼠小腸上皮短肽載體1(PepT1)錶達及功能的影響.方法 雄性SD大鼠80隻,按隨機數字錶法分為正常組、假手術組、膿毒癥組和Ghrelin榦預組4組,每組20隻.採用盲腸結扎穿孔術(GLP)製備膿毒癥大鼠模型;製模後即靜脈給予Ghrelin榦預.各組術後20 h隨機取10隻大鼠,進行小腸黏膜病理觀察,實時定量聚閤酶鏈反應(PCR)和蛋白質免疫印跡試驗分彆檢測PepT1的mRNA和蛋白錶達,同時檢測小腸上皮PepT1對底物Gly-Sar的攝取濃度;另10隻大鼠記錄7d生存率.結果 與正常組和假手術組相比,膿毒癥組小腸黏膜受損明顯,PepT1 mRNA錶達(21.43±1.37比23.29±1.24、23.23±1.48)、蛋白錶達(7832.55±699.74比13 584.74±360.66、13 152.51±605.72)以及Gly-Sar濃度(60 min:3.23±0.30比6.67±0.51、6.52±0.47)均明顯降低(均P<0.05);與膿毒癥組比較,Ghrelin榦預組大鼠小腸黏膜損傷較輕,生存率(40%比20%)、PepT1 mRNA錶達(22.73±1.16比21.43±1.37)、蛋白錶達(10 506.26±850.59比7832.55±699.74)及Gly-Sar濃度(60 min:4.83±0.32比3.23±0.30)均顯著升高(均P<0.05).正常組與假手術組各指標比較無顯著差異.結論 膿毒癥大鼠小腸上皮PepT1mRNA及蛋白錶達明顯下降,機體在基因及蛋白水平下調瞭小腸上皮PepT1生物學功能;Ghrelin榦預對膿毒癥小腸上皮PepT1 mRNA、蛋白錶達以及攝取能力均有上調作用.
목적 탐토뇌장태Ghrelin대농독증대서소장상피단태재체1(PepT1)표체급공능적영향.방법 웅성SD대서80지,안수궤수자표법분위정상조、가수술조、농독증조화Ghrelin간예조4조,매조20지.채용맹장결찰천공술(GLP)제비농독증대서모형;제모후즉정맥급여Ghrelin간예.각조술후20 h수궤취10지대서,진행소장점막병리관찰,실시정량취합매련반응(PCR)화단백질면역인적시험분별검측PepT1적mRNA화단백표체,동시검측소장상피PepT1대저물Gly-Sar적섭취농도;령10지대서기록7d생존솔.결과 여정상조화가수술조상비,농독증조소장점막수손명현,PepT1 mRNA표체(21.43±1.37비23.29±1.24、23.23±1.48)、단백표체(7832.55±699.74비13 584.74±360.66、13 152.51±605.72)이급Gly-Sar농도(60 min:3.23±0.30비6.67±0.51、6.52±0.47)균명현강저(균P<0.05);여농독증조비교,Ghrelin간예조대서소장점막손상교경,생존솔(40%비20%)、PepT1 mRNA표체(22.73±1.16비21.43±1.37)、단백표체(10 506.26±850.59비7832.55±699.74)급Gly-Sar농도(60 min:4.83±0.32비3.23±0.30)균현저승고(균P<0.05).정상조여가수술조각지표비교무현저차이.결론 농독증대서소장상피PepT1mRNA급단백표체명현하강,궤체재기인급단백수평하조료소장상피PepT1생물학공능;Ghrelin간예대농독증소장상피PepT1 mRNA、단백표체이급섭취능력균유상조작용.
Objective To investigate the effect of Ghrelin on expression and function of PepT1 of the small intestinal epithelium in rats with sepsis.Methods Eighty male Sprague-Dawley (SD) rats were divided into four groups by random number table:normal group,sham operation group,sepsis group and Ghrelin group,with 20 rats in each group.The model of sepsis was reproduced with cecal ligation and puncture (CLP).After the procedure Ghrelin was injected via vein in the Ghrelin group.Ten rats in each group were used to study the intestinal pathology.In addition,real time polymerase chain reaction (PCR) and Western blotting were used to detect PepT1 mRNA expression and PepT1 protein expression levels respectively in each group.The uptake of PepT1 by small intestinal epithelial cells was also measured.Seven-day survival was observed in other 10 rats of groups.Results Compared with normal and sham operation groups,the damage to the small intestine mucosa was more serious in sepsis group,and the PepT1 mRNA expression (21.43 ± 1.37 vs.23.29 ± 1.24,23.23 ± 1.48),the PepT1 protein expression (7832.55 ± 699.74 vs.13 584.74 ± 360.66,13 152.51 ± 605.72) and the uptake of PepT1 (60 minutes:3.23 ± 0.30 vs.6.67 ± 0.51,6.52 ± 0.47) in the sepsis group were significantly lowered (all P<0.05).Compared with the sepsis group,less damage to the small intestine mucosa was found in the Ghrelin group,and the survival rate of rats (40% vs.20%),PepT1 mRNA expression (22.73 ± 1.16 vs.21.43 ± 1.37),PepT1 protein expression (10 506.26 ± 850.59 vs.7832.55 ± 699.74) and the uptake of PepT1 (60 minutes:4.83 ± 0.32 vs.3.23 ± 0.30) were significantly improved in the Ghrelin group (all P< 0.05).There was no difference in various indicators between sham operation and normal groups.Conclusions The PepT1 mRNA and protein expression of the small intestinal epithelium in septic rats were significantly decreased,which affected the physiological function of PepT1.Ghrelin could marked up-regulate the PepT1 mRNA and protein expression and uptake of PepT1 of small intestinal epithelial cells.