CAJ | 학술논문

Desmodesmus sp.S-1藻株经重离子诱变后得到的一株突变体 D90G-19,较其野生型的油脂产率提高了20.6%；同时对野生型和突变株的光合放氧速率,色素组成和叶绿素荧光动力学进行了分析。与野生型相比,突变体D90G-19的光合作用有如下特点：1. D90G-19的光饱和点为500μmol/(m2·s),无论是在强光还是在弱光下, D90G-19的光合作用效率都显著高于其野生型；2.突变体D90G-19对高温的适应性比野生型强；3.在弱碱性条件下,D90G-19的光合效率的提升较之野生型更为显著。培养D90G-19的最佳光照强度应在250~400μmol/(m2·s)之间,最适温度在25~30℃,培养液pH值则以弱碱性为宜(7<pH≤8)。叶绿素荧光动力学数据表明,突变体D90G-19的光合作用系统在强光下的损伤程度小于野生型,能够耐受更高的光强胁迫。
Desmodesmus sp.S-1조주경중리자유변후득도적일주돌변체 D90G-19,교기야생형적유지산솔제고료20.6%；동시대야생형화돌변주적광합방양속솔,색소조성화협록소형광동역학진행료분석。여야생형상비,돌변체D90G-19적광합작용유여하특점：1. D90G-19적광포화점위500μmol/(m2·s),무론시재강광환시재약광하, D90G-19적광합작용효솔도현저고우기야생형；2.돌변체D90G-19대고온적괄응성비야생형강；3.재약감성조건하,D90G-19적광합효솔적제승교지야생형경위현저。배양D90G-19적최가광조강도응재250~400μmol/(m2·s)지간,최괄온도재25~30℃,배양액pH치칙이약감성위의(7<pH≤8)。협록소형광동역학수거표명,돌변체D90G-19적광합작용계통재강광하적손상정도소우야생형,능구내수경고적광강협박。
After induced by heavy-ion beam, a mutant of Desmodesmus sp., D90G-19 was obtained, which had a lipid productivity 20.6% higher than the wild type. The photosynthetic characteristics of mutant D90G-19 was characterized by analysis of photosynthetic oxygen evolution rate, chlorophyll concentration and chlorophyll fluo-rescence. Compared to the wild type, the D90G-19 had a higher photosynthetic efficiency regardless of high or low light intensity, the D90G-19 had a higher temperature tolerance and the D90G-19 had a more significant increase in photosynthetic efficiency under weak base condition. The optimum light intensity, temperature and pH for D90G-19 were 250-400μmol/(m2·s), 25-30℃ and 7<pH≤8 respectively. The photo saturation point of D90G-19 was about 500μmol/(m2·s), and the Y(NO) suggested that the damage of photosynthesis system II in D90G-19 was less than that in wild type when they were exposed to high intensity light.