CAJ | 학술논문

Lhx1基因对于哺乳动物卵巢缪勒氏管的形成具有重要作用，但鱼类中的报道仅限于其在体轴和肾脏形成中的作用，尚未见到其与性别及性腺发育相关的报道。本研究克隆获得了牙鲆(Paralichthys olivaceus) Lhx1a和Lhx1b开放阅读框(ORF)序列，长度分别为1224 bp和1206 bp (GenBank注册号为：JX999940、JX999941)，同源序列比较显示牙鲆Lhx1a和Lhx1b基因均具有两个LIM及一个HOX结构域，符合Lhx1进化保守性。这两个基因在牙鲆雌、雄成鱼各组织RT-PCR差异表达谱不尽相同， Lhx1a在精巢中弱表达，卵巢中不表达，在其他组织中雌、雄表达谱差异不大，都只在脑和眼组织有弱表达；而Lhx1b的表达在性腺中相对较高，且卵巢的表达量高于精巢。进一步检测牙鲆Lhx1a和Lhx1b在雌、雄性腺发育各期的表达谱，发现这两个基因表达集中于Ⅲ、Ⅳ期性腺， Lhx1a在精巢中弱表达， Lhx1b卵巢中的表达明显高于精巢，而在Ⅰ、Ⅱ、Ⅱ、Ⅴ期性腺几乎均不表达。由此推断牙鲆 Lhx1a 和 Lhx1b均为性别相关基因，但在两性性腺发育中的作用可能各有不同。
Lhx1기인대우포유동물란소무륵씨관적형성구유중요작용，단어류중적보도부한우기재체축화신장형성중적작용，상미견도기여성별급성선발육상관적보도。본연구극륭획득료아평(Paralichthys olivaceus) Lhx1a화Lhx1b개방열독광(ORF)서렬，장도분별위1224 bp화1206 bp (GenBank주책호위：JX999940、JX999941)，동원서렬비교현시아평Lhx1a화Lhx1b기인균구유량개LIM급일개HOX결구역，부합Lhx1진화보수성。저량개기인재아평자、웅성어각조직RT-PCR차이표체보불진상동， Lhx1a재정소중약표체，란소중불표체，재기타조직중자、웅표체보차이불대，도지재뇌화안조직유약표체；이Lhx1b적표체재성선중상대교고，차란소적표체량고우정소。진일보검측아평Lhx1a화Lhx1b재자、웅성선발육각기적표체보，발현저량개기인표체집중우Ⅲ、Ⅳ기성선， Lhx1a재정소중약표체， Lhx1b란소중적표체명현고우정소，이재Ⅰ、Ⅱ、Ⅱ、Ⅴ기성선궤호균불표체。유차추단아평 Lhx1a 화 Lhx1b균위성별상관기인，단재량성성선발육중적작용가능각유불동。
LIM homeobox protein 1 gene (Lhx1), was reported to play an important role in mammal mullerian duct development. But there is no report on this gene in fish so far, and only few papers studied its function during the formation of axis and kidney. In this study, the open reading frames of Lhx1a and Lhx1b were cloned from olive flounder, Paralichthys olivaceus. Their lengths are 1224 bp (GenBank Accession number, JX999940) and 1206 bp (JX999941), respectively. LHX1a and LHX1b proteins both have two LIM and one HOX highly conserved domains. The different expression patterns of Lhx1a and Lhx1b genes in male and female flounder tissues measured by RT-PCR showed that Lhx1a was expressed in testis but not in ovary. There are almost no expression signals in other tissues, and only brain and eye samples from both male and female individuals showed lower expression signals. The expression of Lhx1b in gonads was totally opposite to that of Lhx1a. Its expression in ovary is higher and that in testis is lower. Moreover, the expression patterns of these two genes at different gonad developmental stages were studied by RT-PCR. The results showed that there were almost no signals for these two genes at stageⅠ, ⅡandⅤin gonads. But, at stageⅢand Ⅳ, the expression could be detected. Lhx1a has a lower expression at stageⅢandⅣof testis tissues, and Lhx1b has a higher expression in ovary and lower expression in testis. Then, both genes were sex-related genes, and their possible roles in testis and ovary development might be different.