中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
18期
2939-2944
,共6页
实验动物%组织构建%体外受精%胚胎移植%不孕%胚胎%体外培养%低温保存%促排卵%临床妊娠率
實驗動物%組織構建%體外受精%胚胎移植%不孕%胚胎%體外培養%低溫保存%促排卵%臨床妊娠率
실험동물%조직구건%체외수정%배태이식%불잉%배태%체외배양%저온보존%촉배란%림상임신솔
fertilization in vitro%embryo transfer%cryopreservation%pregnancy%reproductive technology%auxiliary
背景：从第一例试管婴儿诞生至今已二十余年，体外受精-胚胎移植技术在世界范围内得到蓬勃发展。<br> 目的：回顾性分析体外受精-胚胎移植的助孕效果，探讨体外受精胚胎的培养及低温保存技术的应用。<br> 方法：2013年泸州医学院附属医院生殖医学技术部接受体外受精-胚胎移植或卵细胞浆内单精子注射助孕的不孕患者131例，共147个取卵周期，124个移植周期，患者平均年龄31.32岁。有131个取卵周期应用标准长方案促排卵，4个取卵周期应用超长方案促排卵、10个取卵周期应用超短方案促排卵，2个取卵周期为自然周期排卵；应用新鲜胚胎移植93例，冷冻胚胎移植28例。观察不同促排卵方案、新鲜或冷冻胚胎移植患者的临床妊娠率、胚胎植入率和流产率等。<br> 结果与结论：应用标准长方案、超长方案及超短方案促排卵患者的临床妊娠率为34.7%，50%，20%；植入率分别为19.1%，22.2%，30%；流产率为21.7%，50%，33.3%；以上指标自然周期排卵患者均为0。新鲜胚胎移植临床妊娠率33.6%，植入率18.1%，流产率20.1%；冷冻胚胎移植临床妊娠率53.8%，植入率22.3%，流产率20.7%。提示应用标准长方案促排卵效果好，新鲜及冷冻胚胎移植的疗效相当，胚胎的体外培养及低温冷冻是辅助生殖技术中重要步骤，需进一步深入研究。
揹景：從第一例試管嬰兒誕生至今已二十餘年，體外受精-胚胎移植技術在世界範圍內得到蓬勃髮展。<br> 目的：迴顧性分析體外受精-胚胎移植的助孕效果，探討體外受精胚胎的培養及低溫保存技術的應用。<br> 方法：2013年瀘州醫學院附屬醫院生殖醫學技術部接受體外受精-胚胎移植或卵細胞漿內單精子註射助孕的不孕患者131例，共147箇取卵週期，124箇移植週期，患者平均年齡31.32歲。有131箇取卵週期應用標準長方案促排卵，4箇取卵週期應用超長方案促排卵、10箇取卵週期應用超短方案促排卵，2箇取卵週期為自然週期排卵；應用新鮮胚胎移植93例，冷凍胚胎移植28例。觀察不同促排卵方案、新鮮或冷凍胚胎移植患者的臨床妊娠率、胚胎植入率和流產率等。<br> 結果與結論：應用標準長方案、超長方案及超短方案促排卵患者的臨床妊娠率為34.7%，50%，20%；植入率分彆為19.1%，22.2%，30%；流產率為21.7%，50%，33.3%；以上指標自然週期排卵患者均為0。新鮮胚胎移植臨床妊娠率33.6%，植入率18.1%，流產率20.1%；冷凍胚胎移植臨床妊娠率53.8%，植入率22.3%，流產率20.7%。提示應用標準長方案促排卵效果好，新鮮及冷凍胚胎移植的療效相噹，胚胎的體外培養及低溫冷凍是輔助生殖技術中重要步驟，需進一步深入研究。
배경：종제일례시관영인탄생지금이이십여년，체외수정-배태이식기술재세계범위내득도봉발발전。<br> 목적：회고성분석체외수정-배태이식적조잉효과，탐토체외수정배태적배양급저온보존기술적응용。<br> 방법：2013년로주의학원부속의원생식의학기술부접수체외수정-배태이식혹란세포장내단정자주사조잉적불잉환자131례，공147개취란주기，124개이식주기，환자평균년령31.32세。유131개취란주기응용표준장방안촉배란，4개취란주기응용초장방안촉배란、10개취란주기응용초단방안촉배란，2개취란주기위자연주기배란；응용신선배태이식93례，냉동배태이식28례。관찰불동촉배란방안、신선혹냉동배태이식환자적림상임신솔、배태식입솔화유산솔등。<br> 결과여결론：응용표준장방안、초장방안급초단방안촉배란환자적림상임신솔위34.7%，50%，20%；식입솔분별위19.1%，22.2%，30%；유산솔위21.7%，50%，33.3%；이상지표자연주기배란환자균위0。신선배태이식림상임신솔33.6%，식입솔18.1%，유산솔20.1%；냉동배태이식림상임신솔53.8%，식입솔22.3%，유산솔20.7%。제시응용표준장방안촉배란효과호，신선급냉동배태이식적료효상당，배태적체외배양급저온냉동시보조생식기술중중요보취，수진일보심입연구。
BACKGROUND:Since the first successful birth of a"test tube baby", in vitro fertilization-embryo transfer technology has been greatly developed within 20 years. <br> OBJECTIVE:To retrospectively analyze the assisted reproductive effects of in vitro fertilization-embryo transfer, and to explore the culture of embryos in vitro and the application of cryopreservation technology. <br> METHODS:A total of 131 infertile patients received in vitro fertilization-embryo transfer or intracytoplasmic sperm injection in Department of Reproductive Medicine, Affiliated Hospital of Luzhou Medical Col ege, China. There were 147 oocyte retrieval cycles and 124 transfer cycles, the mean age of the patients was 31.32 years. Standard long-protocol ovulation induction was given to 131 oocyte retrieval cycles, super-long-protocol ovulation induction was given to 4 oocyte retrieval cycles, and super-short-protocol ovulation induction was given to two oocyte retrieval cycles;93 cases were transplanted with fresh embryos and 28 cases with frozen embryos. The clinical pregnancy rate, embryo implantation rate and abortion rate of patients receiving different ovulation induction protocols and fresh or frozen embryo transfer were observed. <br> RESULTS AND CONCLUSION:In the patients receiving standard long, super-long and super-short protocols for ovulation induction, the clinical pregnancy rate was respectively 34.7%, 50%, 20%;the embryo implantation rates were 19.1%, 22.2%, 30%;and abortion rate was 21.7%, 50%, 33.3%;in those patients, no case exhibited natural cycle of ovulation. In the patients receiving fresh embryo transfer, the clinical pregnancy rate was 33.6%, embryo implantation rate was 18.1%, and abortion rate was 20.1%;in frozen embryo transferred patients, the clinical pregnancy rate was 53.8%, embryo implantation rate was 22.3%, and abortion rate was 20.7%. Experimental findings indicate that, standard long-protocol ovulation induction has good effects, the efficacy of fresh embryo transfer is equivalent to that of frozen embryo transfer, in vitro culture and cryopreservation of the embryos are important steps in assisted reproductive technology and deserver further study.
