CAJ | 학술논문

建立了简单、快速、灵敏、节约的饲料中尼卡巴嗪含量的高效液相色谱色谱-单极质谱检测方法。分析物经二甲基亚砜（DMSO）-乙腈（ACN）（4∶6，体积比）混合溶剂提取，氯化钠、硫酸镁和C18粉末净化，冷冻高速离心。采用梯度洗脱。外标法定量。在饲料中添加0.5、1.0、1.5 mg/kg尼卡巴嗪进行重复性试验，结果表明，尼卡巴嗪回收率在93.7%~104.9%之间，相对标准偏差（RSD）不大于3.9%（n=6）。饲料中其它常用的抗球虫药如地克珠利、妥曲珠利及其代谢物等对该检测无干扰。
건립료간단、쾌속、령민、절약적사료중니잡파진함량적고효액상색보색보-단겁질보검측방법。분석물경이갑기아풍（DMSO）-을정（ACN）（4∶6，체적비）혼합용제제취，록화납、류산미화C18분말정화，냉동고속리심。채용제도세탈。외표법정량。재사료중첨가0.5、1.0、1.5 mg/kg니잡파진진행중복성시험，결과표명，니잡파진회수솔재93.7%~104.9%지간，상대표준편차（RSD）불대우3.9%（n=6）。사료중기타상용적항구충약여지극주리、타곡주리급기대사물등대해검측무간우。
A simple, rapid,sensitive and cheaper HPLC -MSD method had been developed for the determination of nicarbazin (4,4'-dintrocarbanilide, DNC) in feedstuffs. The analytes were extracted by dimethylsulfoxide (DMSO)-acetonitrile(4∶6,v/v) and cleaned up by sodium chloride, magnesium sulfate and C18 powder. The quantification was performed with the external standard method. The recoveries of DNC were within 93.7%and 104.9%with spiked level of 0.5, 1.0, 1.5 mg/kg, the RSD was not more than 3.9%(n=6). There was no interference from other Anticoccidials drugs in feeds such as diclazuril , toltrazuril and its metabolites in the determination of nicarbazin.