CAJ | 학술논문

建立一种QuEChERS-超高效液相色谱-串联质谱（QuEChERS-UPLC-MS/MS）检测畜禽组织中金刚烷胺残留的方法。样品用1%乙酸乙腈溶液超声提取，提取液加入C18和PSA填料进行基质分散固相净化，浓缩后经Proshell 120 EC-C18柱分离，用电喷雾离子源正离子多反应监测（MRM）模式进行检测。在0.2μg/L～100.0μg/L线性范围内具有较好的线性关系，相关系数大于0.997。该方法检出限（LOD）为0.6μg/kg，定量限（LOQ）为2.0μg/kg，回收率为70.5%～100.1%，相对标准偏差（RSD）小于10%。该方法简便快速、准确度好，适用于畜禽组织中金刚烷胺药物残留的检测。
건립일충QuEChERS-초고효액상색보-천련질보（QuEChERS-UPLC-MS/MS）검측축금조직중금강완알잔류적방법。양품용1%을산을정용액초성제취，제취액가입C18화PSA전료진행기질분산고상정화，농축후경Proshell 120 EC-C18주분리，용전분무리자원정리자다반응감측（MRM）모식진행검측。재0.2μg/L～100.0μg/L선성범위내구유교호적선성관계，상관계수대우0.997。해방법검출한（LOD）위0.6μg/kg，정량한（LOQ）위2.0μg/kg，회수솔위70.5%～100.1%，상대표준편차（RSD）소우10%。해방법간편쾌속、준학도호，괄용우축금조직중금강완알약물잔류적검측。
A method for the determination of the residues of amantadine in livestock and poultry tissues by QuEChERS extraction and ultra high performance liquid chromatograph-tandem mass spectrometric method (UPLC-MS/MS) was established. The sample was extracted with acetonitrile (containing 1%acetic acid) and followed by ultrasound. Cleanup of the extracts was processed by C18 and PSA , a modified dispersive solid phase extraction (QuEChERS). The analysts were then separated by Poroshell 120 EC-C18 column, and finally detected by tandem mass spectrometry in positive ESI mode. The linearity of amantadine in the range from 0.2 μg/kg to 100.0μg/kg had correlation coefficient greater than 0.997. The limits of detection (LOD) of the method were 0.6μg/kg and the limits of quantification (LOQ) were 2.0μg/kg. The average recoveries of amantadine were in the range from 70.5%to 100.1%, with relative standard deviations were less than 10%.The established method is sensitive and of good recoveries. It applies to the determination of the residues of amantadine in poultry tissues and meat.