CAJ | 학술논문

建立动物组织中4种硝基呋喃代谢物残留量的聚合物整体柱微萃取和超高效液相色谱-串联质谱检测方法。以聚甲基丙烯酸-2乙二醇二甲基丙烯酸酯毛细管整体柱作为萃取装置。为了得到较高的萃取效率，优化了影响萃取效率的参数：萃取流速、萃取体积、样品pH。样品经过匀浆、提取、磷酸盐缓冲溶液稀释、离心等步骤后直接进行萃取。动物组织中四种硝基呋喃代谢物的检出限为0.5μg/kg，在0.5μg/kg～10μg/kg的浓度范围内具有良好的线性关系。加标回收率大于65%，日内、日间测定的相对标准偏差不高于81.2%。结果表明，方法简单、快速、灵敏度高，适用于动物组织中四种硝基呋喃代谢物的常规分析。
건립동물조직중4충초기부남대사물잔류량적취합물정체주미췌취화초고효액상색보-천련질보검측방법。이취갑기병희산-2을이순이갑기병희산지모세관정체주작위췌취장치。위료득도교고적췌취효솔，우화료영향췌취효솔적삼수：췌취류속、췌취체적、양품pH。양품경과균장、제취、린산염완충용액희석、리심등보취후직접진행췌취。동물조직중사충초기부남대사물적검출한위0.5μg/kg，재0.5μg/kg～10μg/kg적농도범위내구유량호적선성관계。가표회수솔대우65%，일내、일간측정적상대표준편차불고우81.2%。결과표명，방법간단、쾌속、령민도고，괄용우동물조직중사충초기부남대사물적상규분석。
A method of detecting four kinds of nitrofuran metabolites in animal tissues by polymer monolith microextraction and ultra performance liquid chromatography-tandem mass spectrometry was established in this study. The polymethyl methacrylate-2 two glycol methacrylate monolithic capillary column was used as the extraction device, and the extracting conditions, which were extraction volume, flow rate and pH value of the sample matrix, were optimized to improve the extraction efficiency. The samples were extracted after being homogenized, distilled, diluting with phosphate buffer solution, and centrifugating. The results showed that, the detection limit of these four kinds of nitrofuran metabolites in animal tissues is 0.5μg/kg, with a better linear relationship in the concentration range of 0.5 μg/kg-10 μg/kg. The recovery of standard addition was above 65 %, and the relative standard deviation was lower than 81.2%. The detecting method given in this study , exhibited three advantages of simplicity, rapid, and high sensitivity, was suitable for the routine analysis of four kinds of nitrofuran metabolites in animal tissues.