CAJ | 학술논문

以丁二酮（Diacetyl）为底物，以面包酵母（Saccharomyces cerevisiae）休止细胞为还原剂，选择性合成（S）-乙偶姻。分别考察了不同菌株、辅助底物、辅助底物浓度、缓冲液体系pH值和酶抑制剂等因素对丁二酮转化率和（S）-乙偶姻ee值的影响。结果表明：丁二酮的转化（S）-乙偶姻的适宜条件为：面包酵母XG-4-2，辅助底物为30.0 g/L乙醇，0.05 mol/L pH 5.0的Na2HPO4-KH2PO4缓冲体系，酶抑制剂异丁醇加量为2.0 g/L。丁二酮转化率可达82%，（S）-乙偶姻纯度可达到91.2%，（S）-乙偶姻对映体过量值（ee值）达82.4%。
이정이동（Diacetyl）위저물，이면포효모（Saccharomyces cerevisiae）휴지세포위환원제，선택성합성（S）-을우인。분별고찰료불동균주、보조저물、보조저물농도、완충액체계pH치화매억제제등인소대정이동전화솔화（S）-을우인ee치적영향。결과표명：정이동적전화（S）-을우인적괄의조건위：면포효모XG-4-2，보조저물위30.0 g/L을순，0.05 mol/L pH 5.0적Na2HPO4-KH2PO4완충체계，매억제제이정순가량위2.0 g/L。정이동전화솔가체82%，（S）-을우인순도가체도91.2%，（S）-을우인대영체과량치（ee치）체82.4%。
(S)-acetoin was synthesized selectively using diacetyl as substrate and baker yeast resting cells as reductase. The effect of bacteria, cosubstrate, cosubstrate concentration, pH of buffer system and enzyme inhibitors on the transformation of diacetyl and ee value of (S)-acetoin was studied. The result showed the optimal conditions were:Saccharomyces cerevisiae XG-4-2, cosubstrate (ethanol)30 g/L, buffer system:0.05 mol/L Na2HPO4-KH2PO4 (pH5.0), substrate 2 g/L, enzyme inhibitor (isopropanol) 2 g/L. The transformation rate of diacetyl was 82 %, in which(S)-acetoin was covered with 91.2%and ee value of(S)-acetoin was 82.4%.