医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
9期
154-155
,共2页
脑外伤%骨折%骨痂%低氧诱导因子%HIF-1
腦外傷%骨摺%骨痂%低氧誘導因子%HIF-1
뇌외상%골절%골가%저양유도인자%HIF-1
Brain injury%Osteotylus%Fracture%Hypoxia-inducible factor%HIF-1
目的：探讨脑外伤合并骨折组与单纯骨折组骨折处骨痂组织中低氧诱导因子（HIF-1）的表达。方法对98只SPF级健康SD大鼠进行造模，其中A组（脑外伤合并骨折组）50例，B组（单纯骨折组）48例，造模成功后分别在1~4w每周周末剖取骨痂组织进行HE染色和免疫组化染色。利用高倍显微镜观察组织中4个互不重叠视野，计算细胞HIF-1阳性率，灰度值、光密度值、积分光密度值，观测细胞HIF-1的表达。结果 A组的各种指标均在第1w末达到高峰后开始换面下降，说明HIF-1在脑外伤合并骨折后增多，其后虽下降但任然活跃，加速骨折愈合。而单纯骨折组所有指标均无明显变化。结论脑外伤合并骨折组的骨痂组织中HIF-1的表达较单纯骨折组有明显增多。 HIF-1对骨折愈合有促进作用。这为日后临床治疗骨折愈合，提高治疗效率提供新方向和新靶点。
目的：探討腦外傷閤併骨摺組與單純骨摺組骨摺處骨痂組織中低氧誘導因子（HIF-1）的錶達。方法對98隻SPF級健康SD大鼠進行造模，其中A組（腦外傷閤併骨摺組）50例，B組（單純骨摺組）48例，造模成功後分彆在1~4w每週週末剖取骨痂組織進行HE染色和免疫組化染色。利用高倍顯微鏡觀察組織中4箇互不重疊視野，計算細胞HIF-1暘性率，灰度值、光密度值、積分光密度值，觀測細胞HIF-1的錶達。結果 A組的各種指標均在第1w末達到高峰後開始換麵下降，說明HIF-1在腦外傷閤併骨摺後增多，其後雖下降但任然活躍，加速骨摺愈閤。而單純骨摺組所有指標均無明顯變化。結論腦外傷閤併骨摺組的骨痂組織中HIF-1的錶達較單純骨摺組有明顯增多。 HIF-1對骨摺愈閤有促進作用。這為日後臨床治療骨摺愈閤，提高治療效率提供新方嚮和新靶點。
목적：탐토뇌외상합병골절조여단순골절조골절처골가조직중저양유도인자（HIF-1）적표체。방법대98지SPF급건강SD대서진행조모，기중A조（뇌외상합병골절조）50례，B조（단순골절조）48례，조모성공후분별재1~4w매주주말부취골가조직진행HE염색화면역조화염색。이용고배현미경관찰조직중4개호불중첩시야，계산세포HIF-1양성솔，회도치、광밀도치、적분광밀도치，관측세포HIF-1적표체。결과 A조적각충지표균재제1w말체도고봉후개시환면하강，설명HIF-1재뇌외상합병골절후증다，기후수하강단임연활약，가속골절유합。이단순골절조소유지표균무명현변화。결론뇌외상합병골절조적골가조직중HIF-1적표체교단순골절조유명현증다。 HIF-1대골절유합유촉진작용。저위일후림상치료골절유합，제고치료효솔제공신방향화신파점。
Objective The study set up traumatic brain injury associated with bone fracture rat model and bone fracture rat model. By HE staining and immunohistochemistry staining to detect the expression of HIF-1 in osteotylus. Methods:98 specified-pathogens free adult SD rats were divided into two groups randomly:(1)traumatic brain injury associated with fracture group(A,n=50);(2)fracture group(B,n=48). After set up model successful y,rats were kil ed respectively on 1st,2nd,3rd,4th week and intercepted osteotylus specimens from the fracture upper and lower about 5mm.Detect the expression of HIF-1 and analyes dates statictical y. Results: HIF-1 shows expression up-regulation in osteotylus specimens of A group, compared with B group, the results have statistical significance.It show that the healing speed of A group were much faster than B group.HIF-1 expression much large in 1st and decrease after that. While B group have no marked change on that. Conclusion:HIF-1 shows the expression up-regulatuion in cal us of fraeture sites in traumatic brain injury associated with bone fracture rat model. Compared with simple bone fracture rat model,the result show that HIF-1 can accelerated the healing of fracture.
