CAJ | 학술논문

该文研究棕榈油中间熔点物（POMF）与硬脂酸（StA）进行酶酯交换制备类可可脂。利用胰脂酶定向水解和Lipozyme TL IM脂肪酶酯交换的特性，结合TLC和GC法分析Sn-2位酰基转移率和酯交换的程度。从而得出棕榈油中间物制备类可可脂的反应条件：POMF为5 g，水活度为0.43，加酶量为10%，底物质量比为1∶1.8，振荡速度为150 r/min，反应温度60℃，反应时间10 h。此时，酯交换程度为75.70%，酰基转移率为12.30%，产品的滑动熔点为31~35℃，酸值为1.2 mgKOH/g。
해문연구종려유중간용점물（POMF）여경지산（StA）진행매지교환제비류가가지。이용이지매정향수해화Lipozyme TL IM지방매지교환적특성，결합TLC화GC법분석Sn-2위선기전이솔화지교환적정도。종이득출종려유중간물제비류가가지적반응조건：POMF위5 g，수활도위0.43，가매량위10%，저물질량비위1∶1.8，진탕속도위150 r/min，반응온도60℃，반응시간10 h。차시，지교환정도위75.70%，선기전이솔위12.30%，산품적활동용점위31~35℃，산치위1.2 mgKOH/g。
In the paper,the production of cocoa butter equivalent by enzymatic interesterification of palm oil midfraction(POMF)and stearic acid(StA)was studied. The characteristics of directed hydrolysis of pancreatic lipase and Lipozyme TL IM catalyzed interesterification were utilized. Sn-2 acyl migration rate and ester exchange degree were analyzed in combination with thin-layer chromatography and gas chromatography. The reaction conditions of cocoa butter equivalent production were as follows:POMF was 5 g,water activity was 0.43,the amount of enzyme was 10%,the mass ratio of POMF to StA was 1∶1.8,shaking speed was 150 r/min,the reaction temperature was 60℃and the reaction time was 10 h. Under the circumstances,the ester exchange degree was 75.70%,the acyl migration rate was 12.30%,the slip melting point of the production was 31~35℃and the acid value was 1.2 mgKOH/g.