CAJ | 학술논문

目的：研究白细胞介素（IL）-17对系膜细胞增殖及分泌功能的影响，并探讨其可能的分子机制。方法四甲基偶氮唑盐比色（MTT）法检测IL-17对系膜细胞增殖的影响；酶联免疫法（ELISA）检测IL-17对系膜细胞分泌IL-1β的影响；免疫细胞化学法检测系膜细胞NF-κB的表达。结果不同浓度IL-17在不同时间点对系膜细胞增殖的影响均无统计学意义（P>0.05）；IL-17可促进系膜细胞分泌IL-1β，且呈浓度时间依赖性，差异有统计学意义（P<0.05）；同时IL-17诱导组NF-κB的表达高于空白对照组，差异有统计学意义（P<0.05）。结论 IL-17可能部分通过NF-κB途径引起系膜细胞分泌炎症因子IL-1β，加重肾脏局部的炎症状态，导致肾脏损伤。
목적：연구백세포개소（IL）-17대계막세포증식급분비공능적영향，병탐토기가능적분자궤제。방법사갑기우담서염비색（MTT）법검측IL-17대계막세포증식적영향；매련면역법（ELISA）검측IL-17대계막세포분비IL-1β적영향；면역세포화학법검측계막세포NF-κB적표체。결과불동농도IL-17재불동시간점대계막세포증식적영향균무통계학의의（P>0.05）；IL-17가촉진계막세포분비IL-1β，차정농도시간의뢰성，차이유통계학의의（P<0.05）；동시IL-17유도조NF-κB적표체고우공백대조조，차이유통계학의의（P<0.05）。결론 IL-17가능부분통과NF-κB도경인기계막세포분비염증인자IL-1β，가중신장국부적염증상태，도치신장손상。
Objectives To investigate the effect of IL-17 on proliferation and secretion of IL-1βin the human mesangial cell line (HMCL) and explore the molecular mechanism. Methods The proliferation of HMCL induced by IL-17 was detected by MTT, the secretion of IL-1βin HMCL was measured by ELISA and the expression of NF-κB was detected by immunocyto-chemistry. Results IL-17 did not impact HMCL proliferation (P>0.05) but dose-and time-dependently promoted the secretion of IL-1β(P<0.05). And the expression of NF-κB induced by IL-17 increased comparing to that in the control group (P<0.05). Con-clusions IL-17 may induce HMCL to secrete IL-1βvia NF-κB pathway that results in kidney injury.