临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
2期
167-169
,共3页
韩启军%温天莲%沈国红%管长丽%窦冬冬
韓啟軍%溫天蓮%瀋國紅%管長麗%竇鼕鼕
한계군%온천련%침국홍%관장려%두동동
人巨细胞病毒%先天性感染%乙肝疫苗%免疫效果%大鼠
人巨細胞病毒%先天性感染%乙肝疫苗%免疫效果%大鼠
인거세포병독%선천성감염%을간역묘%면역효과%대서
human cytomegalovirus%congenital infection%hepatitis B vaccine%immune effect%rat
目的:探讨先天性人巨细胞病毒(HCMV)感染对乙型肝炎(乙肝)疫苗免疫效果的影响。方法孕鼠腹腔注射HCMV悬液,以其子鼠为先天性HCMV感染模型,以健康大鼠的子鼠作为对照组。各组子鼠分别于生后1、3、5周接种乙肝疫苗,并于3、5、7、11周心脏采血,应用酶联免疫分析法检测各组子鼠血清乙肝表面抗体(HBsAb)滴度。结果随接种次数增加,两组子鼠血清HBsAb滴度均呈逐渐增加趋势,接种完成后随时间延长又有不同程度下降,两组子鼠血清HBsAb滴度随时间变化的差异均有统计学意义(P均<0.001)。在各个时间点(3、5、7、11周),先天性HCMV感染组子鼠的血清HBsAb滴度均低于对照组,差异有统计学意义(P均<0.01)。结论先天性HCMV感染可减弱乙肝疫苗免疫效果。
目的:探討先天性人巨細胞病毒(HCMV)感染對乙型肝炎(乙肝)疫苗免疫效果的影響。方法孕鼠腹腔註射HCMV懸液,以其子鼠為先天性HCMV感染模型,以健康大鼠的子鼠作為對照組。各組子鼠分彆于生後1、3、5週接種乙肝疫苗,併于3、5、7、11週心髒採血,應用酶聯免疫分析法檢測各組子鼠血清乙肝錶麵抗體(HBsAb)滴度。結果隨接種次數增加,兩組子鼠血清HBsAb滴度均呈逐漸增加趨勢,接種完成後隨時間延長又有不同程度下降,兩組子鼠血清HBsAb滴度隨時間變化的差異均有統計學意義(P均<0.001)。在各箇時間點(3、5、7、11週),先天性HCMV感染組子鼠的血清HBsAb滴度均低于對照組,差異有統計學意義(P均<0.01)。結論先天性HCMV感染可減弱乙肝疫苗免疫效果。
목적:탐토선천성인거세포병독(HCMV)감염대을형간염(을간)역묘면역효과적영향。방법잉서복강주사HCMV현액,이기자서위선천성HCMV감염모형,이건강대서적자서작위대조조。각조자서분별우생후1、3、5주접충을간역묘,병우3、5、7、11주심장채혈,응용매련면역분석법검측각조자서혈청을간표면항체(HBsAb)적도。결과수접충차수증가,량조자서혈청HBsAb적도균정축점증가추세,접충완성후수시간연장우유불동정도하강,량조자서혈청HBsAb적도수시간변화적차이균유통계학의의(P균<0.001)。재각개시간점(3、5、7、11주),선천성HCMV감염조자서적혈청HBsAb적도균저우대조조,차이유통계학의의(P균<0.01)。결론선천성HCMV감염가감약을간역묘면역효과。
Objectives To investigate the immune effect of hepatitis B vaccine under the influence of congenital human cytomegalovirus (HCMV) infection. Methods The newborn rat model of congenital HCMV infection was developed by intra-peritoneally inoculating pregnant rat with HCMV suspension,while the offsprings of healthy rats were used as the control group. Offspring rats in all groups were inoculated with hepatitis B vaccine in the postnatal 1st, 3rd and 5th week and were taken blood from hearts separately in 3rd, 5th, 7th and 11th week. Antibody to Hepatitis B surface antigen (HBsAb) titer in all groups was de-tected by ELISA method. Results The serum HBsAb titer in both groups all showed a trend of increasing gradually with added vaccinating times and decreased differently with time extending after completed vaccinations. Differences among changes of HBsAb titer along with prolonged time in each group were all statistically significant (P<0.001). At all time points (3rd, 5th, 7th, 11th week), the titer of serum HBsAb in congenital HCMV infection group was lower than that in the control group respectively, and there were statistically significant differences (P<0.01). Conclusions Congenital HCMV infection could weaken the im-mune effect of hepatitis B vaccine.