淮海医药
淮海醫藥
회해의약
JOURNAL OF HUAIHAI MEDICINE
2014年
2期
103-104,105
,共3页
姚廷敬%张晖%彭德峰%朱正志%马小开%周锐%朱金海%崔振%王岩岩
姚廷敬%張暉%彭德峰%硃正誌%馬小開%週銳%硃金海%崔振%王巖巖
요정경%장휘%팽덕봉%주정지%마소개%주예%주금해%최진%왕암암
甲状腺肿瘤%miRNA-30b%纳米粒%转染%细胞生物学
甲狀腺腫瘤%miRNA-30b%納米粒%轉染%細胞生物學
갑상선종류%miRNA-30b%납미립%전염%세포생물학
Thyroid neoplasm%miRNA-30b%Nanoparticles%Transfection%Cytobiology
目的：研究miRNA-30b纳米粒对甲状腺未分化癌细胞株TA-K细胞的生物学特性的影响。方法转染不同浓度的miRNA-30b纳米粒进入TA-K细胞中，通过流式法检测TA-K细胞凋亡情况，后通过生物信息学预测miR-NA-30b的生物学靶点，并通过RT-PCR及Western Blot方法验证。结果发现转染了miRNA-30b纳米粒后，TA-K细胞的凋亡显著增加，而生物信息学显示miRNA-30b可靶向作用于Survivin，后经RT-PCR及Western blot 法证实Sur-vivin确实是miRNA-30b的生物学靶点。结论 miRNA-30b可以通过作用Survivin促进TA-K细胞的凋亡，这一现象也为将来甲状腺未分化癌的临床治疗提供了新的思路。
目的：研究miRNA-30b納米粒對甲狀腺未分化癌細胞株TA-K細胞的生物學特性的影響。方法轉染不同濃度的miRNA-30b納米粒進入TA-K細胞中，通過流式法檢測TA-K細胞凋亡情況，後通過生物信息學預測miR-NA-30b的生物學靶點，併通過RT-PCR及Western Blot方法驗證。結果髮現轉染瞭miRNA-30b納米粒後，TA-K細胞的凋亡顯著增加，而生物信息學顯示miRNA-30b可靶嚮作用于Survivin，後經RT-PCR及Western blot 法證實Sur-vivin確實是miRNA-30b的生物學靶點。結論 miRNA-30b可以通過作用Survivin促進TA-K細胞的凋亡，這一現象也為將來甲狀腺未分化癌的臨床治療提供瞭新的思路。
목적：연구miRNA-30b납미립대갑상선미분화암세포주TA-K세포적생물학특성적영향。방법전염불동농도적miRNA-30b납미립진입TA-K세포중，통과류식법검측TA-K세포조망정황，후통과생물신식학예측miR-NA-30b적생물학파점，병통과RT-PCR급Western Blot방법험증。결과발현전염료miRNA-30b납미립후，TA-K세포적조망현저증가，이생물신식학현시miRNA-30b가파향작용우Survivin，후경RT-PCR급Western blot 법증실Sur-vivin학실시miRNA-30b적생물학파점。결론 miRNA-30b가이통과작용Survivin촉진TA-K세포적조망，저일현상야위장래갑상선미분화암적림상치료제공료신적사로。
Objective To explore the influence of miRNA-30b nanoparticles transaction on biological characteristics of an-aplastic thyroid carcinoma cell line TA-K cells.Methods Different concentrations of miRNA-30b nanoparticles were trans-fected into TA-K cells and apoptosis rate was detected by flow assay .MiRNA-30b biological targets were then predicted through bioinformatics analysis , and validated using RT-PCR and Western Blot methods .Results After miRNA-30b nanoparticles transfection,the apoptosis rate of TA-K cells significantly increased .Bioinformatics showed miRNA-30b could be targeted on Survivin,which was bioinformatically confirmed by RT-PCR and Western blot methods .Conclusion The study indicates that miRNA-30b can promote apoptosis by targeting Survivin in TA-K cells.This phenomenon provides a new approach to anaplastic thyroid carcinoma therapy .
