西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2014年
2期
23-24,25
,共3页
不同采样期%党参%党参炔苷%高效液相
不同採樣期%黨參%黨參炔苷%高效液相
불동채양기%당삼%당삼결감%고효액상
different sampling periods%DangShen%lobetyolin%HPLC
目的：分析不同采样期党参炔苷的含量。方法：炔苷含量采用高效液相色谱分析，TC-C18色谱柱(4.6 mm×250 mm，5μm)，以乙腈-水(22∶78)为流动相,流速：1.0 mL/min。检测波长267 nm，柱温25℃。结果：党参炔苷在1.12～0.80μg/mL范围内呈良好的线性关系，r=0.9996，精密度RSD为1.28%(n=6)，重复性RSD为1.9%，平均回收率为99.50%，RSD为1.90%(n=6)，测定了不同采收期党参中党参炔苷的含量范围为0.87～3.00 mg/g。结论：分析了不同采样期党参炔苷的含量，为党参规范化栽培提供参考。
目的：分析不同採樣期黨參炔苷的含量。方法：炔苷含量採用高效液相色譜分析，TC-C18色譜柱(4.6 mm×250 mm，5μm)，以乙腈-水(22∶78)為流動相,流速：1.0 mL/min。檢測波長267 nm，柱溫25℃。結果：黨參炔苷在1.12～0.80μg/mL範圍內呈良好的線性關繫，r=0.9996，精密度RSD為1.28%(n=6)，重複性RSD為1.9%，平均迴收率為99.50%，RSD為1.90%(n=6)，測定瞭不同採收期黨參中黨參炔苷的含量範圍為0.87～3.00 mg/g。結論：分析瞭不同採樣期黨參炔苷的含量，為黨參規範化栽培提供參攷。
목적：분석불동채양기당삼결감적함량。방법：결감함량채용고효액상색보분석，TC-C18색보주(4.6 mm×250 mm，5μm)，이을정-수(22∶78)위류동상,류속：1.0 mL/min。검측파장267 nm，주온25℃。결과：당삼결감재1.12～0.80μg/mL범위내정량호적선성관계，r=0.9996，정밀도RSD위1.28%(n=6)，중복성RSD위1.9%，평균회수솔위99.50%，RSD위1.90%(n=6)，측정료불동채수기당삼중당삼결감적함량범위위0.87～3.00 mg/g。결론：분석료불동채양기당삼결감적함량，위당삼규범화재배제공삼고。
Objective:To analyze the contents of lobetyolin in DangShen[Codonopsis pilosula(Franch.)Nannf.] at different sampling periods. Methods:The contents of lobetyolin were analyzed by HPLC, TC-C 18 chromatographic column (4.6 mm×250 mm, 5μm), acetonitrile-water (22:78) were as the mobile phase, flow rate:1.0mL/min. Detec-tion wavelength was 267 nm, the temperature: 25℃. Results: Lobetyolin showed better linear relationship in the range between 1.12 to 0.80μg/mL, r=0.999 6, accuracy RSD was 1.28%(n=6), repetition RSD was 1.9%, average recovery rate was 99.50%, RSD was 1.90% (n=9), the contents of lobetyolin ranged from 0.87 to 3.00mg/g at different sampling periods.Conclusion:The contents of lobetyolin are analyzed in different sampling periods in order to provide reference for standardized cultivation of DangShen.
