国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
2期
208-209,212
,共3页
抗坏血酸%尿酸%指示剂和试剂
抗壞血痠%尿痠%指示劑和試劑
항배혈산%뇨산%지시제화시제
ascorbic acid%uric acid%indicators and reagents
目的：分析用尿酸酶法测定尿酸时维生素C与维生素C氧化酶之间的量效关系，为临床尿酸试剂盒的选购提供合理化依据。方法用试剂盒测定维生素C氧化酶的活性，配制含不同浓度维生素C氧化酶的尿酸检测试剂。并以维生素C为干扰物，检测不同浓度维生素C氧化酶的抗干扰能力。结果反应体系中维生素C氧化酶浓度为200 U/L时，对健康人群（维生素C小于或等于0．04 mmol/L）尿酸测定结果没有明显干扰（干扰值-4．2μmol/L＜±1．96犛犇）；当维生素C氧化酶浓度升高至400 U/L时，对使用维生素C 治疗后的人群（维生素C≤0．37 mmol/L）尿酸检测值也不会受到显著影响（干扰值-3．0μmol/L＜±1．96犛犇）。结论在选购尿酸试剂时应选用维生素C氧化酶大于或等于400 U/L，可以有效防止使用维生素C 治疗人群血液中维生素C对检测结果的干扰，使检测值更接近人体血清中真实的尿酸水平。
目的：分析用尿痠酶法測定尿痠時維生素C與維生素C氧化酶之間的量效關繫，為臨床尿痠試劑盒的選購提供閤理化依據。方法用試劑盒測定維生素C氧化酶的活性，配製含不同濃度維生素C氧化酶的尿痠檢測試劑。併以維生素C為榦擾物，檢測不同濃度維生素C氧化酶的抗榦擾能力。結果反應體繫中維生素C氧化酶濃度為200 U/L時，對健康人群（維生素C小于或等于0．04 mmol/L）尿痠測定結果沒有明顯榦擾（榦擾值-4．2μmol/L＜±1．96犛犇）；噹維生素C氧化酶濃度升高至400 U/L時，對使用維生素C 治療後的人群（維生素C≤0．37 mmol/L）尿痠檢測值也不會受到顯著影響（榦擾值-3．0μmol/L＜±1．96犛犇）。結論在選購尿痠試劑時應選用維生素C氧化酶大于或等于400 U/L，可以有效防止使用維生素C 治療人群血液中維生素C對檢測結果的榦擾，使檢測值更接近人體血清中真實的尿痠水平。
목적：분석용뇨산매법측정뇨산시유생소C여유생소C양화매지간적량효관계，위림상뇨산시제합적선구제공합이화의거。방법용시제합측정유생소C양화매적활성，배제함불동농도유생소C양화매적뇨산검측시제。병이유생소C위간우물，검측불동농도유생소C양화매적항간우능력。결과반응체계중유생소C양화매농도위200 U/L시，대건강인군（유생소C소우혹등우0．04 mmol/L）뇨산측정결과몰유명현간우（간우치-4．2μmol/L＜±1．96리분）；당유생소C양화매농도승고지400 U/L시，대사용유생소C 치료후적인군（유생소C≤0．37 mmol/L）뇨산검측치야불회수도현저영향（간우치-3．0μmol/L＜±1．96리분）。결론재선구뇨산시제시응선용유생소C양화매대우혹등우400 U/L，가이유효방지사용유생소C 치료인군혈액중유생소C대검측결과적간우，사검측치경접근인체혈청중진실적뇨산수평。
Objective To analyze the concentration response relationship of ascorbic acid and vitamin C oxidase in determination of serum uric acid based on uricase method.In order to provide references for purchase of clinical uric acid reagents.Methods Dif-ferent concentrations of vitamin C oxidase in uric acid reagents were prepared.The ascorbic acid oxidase activity was detected with kit.The anti-interference ability of vitamin C oxidase at various concentration was determined when ascorbic acid as the interference substance.Results In case of vitamin C oxidase concentration was 200 U/L in reaction system,the effect on detection of uric acid (interference value -4.2μmol/L<±1.96SD)for healthy crowd(ascorbic acid C≤0.04 mmol/L)was inconspicuous;and when vitamin C oxidase concentration increased to 400 U/L,the influence on measure of uric acid (interference value 3 .0μmol/L<± 1.96SD)for the ascorbic acid treating group (ascorbic acid C≤0.37 mmol/L)was unobvious either.Conclusion To prevent the interference of blood ascorbic acid for determination of uric acid in the ascorbic acid treating group and make the value of uric acid closed to true level,the uric acid reagents with vitamin C oxidase concentration ≥400 U/L is supposed to be chosen.
