国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
2期
203-204,207
,共3页
实验室技术和方法%肿瘤标记,生物学%流式荧光发光法
實驗室技術和方法%腫瘤標記,生物學%流式熒光髮光法
실험실기술화방법%종류표기,생물학%류식형광발광법
laboratory techniques and procedures%tumor markers,biological%flow cytometry fluorescence luminescence as-say
目的：评价流式荧光发光法在AFP、CEA、CA125、CA199定量测定中的可靠性。方法在Luminex200多功能流式点阵仪上评价了本法的精密度、准确度（回收实验）、检出限、线性和抗干扰性，并分析了本法与电化学发光法检测结果的相关性。结果批内变异系数均小于10％，批间变异每米均小于13％，回收率在89％～113％之间，灵敏度较好，线性良好（r＞0．99），血红蛋白、胆红素、三酰甘油对测定结果无显著性干扰（P＞0．05），与电化学发光法检测结果相关性良好（r＞0．975）。结论本方法测定结果准确、可靠，操作简便、快速，适宜于临床检测推广。
目的：評價流式熒光髮光法在AFP、CEA、CA125、CA199定量測定中的可靠性。方法在Luminex200多功能流式點陣儀上評價瞭本法的精密度、準確度（迴收實驗）、檢齣限、線性和抗榦擾性，併分析瞭本法與電化學髮光法檢測結果的相關性。結果批內變異繫數均小于10％，批間變異每米均小于13％，迴收率在89％～113％之間，靈敏度較好，線性良好（r＞0．99），血紅蛋白、膽紅素、三酰甘油對測定結果無顯著性榦擾（P＞0．05），與電化學髮光法檢測結果相關性良好（r＞0．975）。結論本方法測定結果準確、可靠，操作簡便、快速，適宜于臨床檢測推廣。
목적：평개류식형광발광법재AFP、CEA、CA125、CA199정량측정중적가고성。방법재Luminex200다공능류식점진의상평개료본법적정밀도、준학도（회수실험）、검출한、선성화항간우성，병분석료본법여전화학발광법검측결과적상관성。결과비내변이계수균소우10％，비간변이매미균소우13％，회수솔재89％～113％지간，령민도교호，선성량호（r＞0．99），혈홍단백、담홍소、삼선감유대측정결과무현저성간우（P＞0．05），여전화학발광법검측결과상관성량호（r＞0．975）。결론본방법측정결과준학、가고，조작간편、쾌속，괄의우림상검측추엄。
Objective To evaluate the reliability of the quantitative determination of tumor markers AFP,CEA,CA125 and CA199 based on the flow cytometry fluorescence method.Methods The precision,accuracy,sensitivity,linearity and interference resistance of the kits were evaluated with the automated analyzed Luminex 200 as determination instrument,and the correlation of the results were analysis with that of electrochemiluminescence.Results The chemical character evaluation showed that the within-run and between-run coefficients of variation(CV)were less than 10% and 13%,respectively.The recovery rate was between 89%-113%,and the sensitivity was high.The results demonstrated excellent linearity(r>0.99).The assay had no significant in-terference by haemoglobin (Hb),bilirubin (Bil)and trig lyceride (TG)(P>0.05).The correlation between the evaluated kits and electrochemiluminescen was excellent (r>0.975).Conclusion The quantitative assay is safe,accurate and easy to operate,and it is worthy to extend in clinical application.
