CAJ | 학술논문

目的：探讨抗心磷脂抗体（ACA）和抗精子抗体（AsAb）在女性不孕诊断中的价值。方法选取2012年1～12月湖南省妇幼保健院确诊为不孕症的200例女性患者及健康体检者100例，采用酶联免疫吸附实验（ELISA）检测2组血清抗精子抗体（AsAb）、抗心磷脂抗体（ACA）并比较不孕症组与对照组2项抗体的阳性率。结果血清2项生殖免疫性抗体阳性者79例，阳性率39．5％；不孕患者组AsAb阳性率25．5％（51/200）与健康对照组 AsAb 阳性率1％（1/100）比较，差异有统计学意义（P＝0．000）；不孕患者组ACA阳性率为24％（48/200）与健康对照组ACA 阳性率1％（1/100）比较，差异有统计学意义（P＝0．000）。AsAb-IgG、AsAb-IgM、AsAb-IgA和ACA-IgM、ACA-IgG阳性率与对照组比较差异均有统计学意义（P＜0．05）；ACA-IgA 阳性率与对照组比较差异无统计学意义（P＞0．05）；AsAb-IgM和ACA-IgG在不孕不育组内与其他类型抗体阳性率比较，差异均有统计学意义（P＜0．05）。AsAb、ACA阳性率在不同年龄段间与对照组比较，3组差异均有统计学意义（P＜0．05）。结论 AsAb、ACA 是引起不孕不育的重要的免疫因素，且AsAb-IgM和ACA-IgG与女性不孕不育的关系更加紧密。这些抗体对不同年龄人群的不孕不育患者均有影响，因此AsAb、ACA 的检测对免疫性因素引起不孕不育患者的诊断和治疗具有临床价值。
목적：탐토항심린지항체（ACA）화항정자항체（AsAb）재녀성불잉진단중적개치。방법선취2012년1～12월호남성부유보건원학진위불잉증적200례녀성환자급건강체검자100례，채용매련면역흡부실험（ELISA）검측2조혈청항정자항체（AsAb）、항심린지항체（ACA）병비교불잉증조여대조조2항항체적양성솔。결과혈청2항생식면역성항체양성자79례，양성솔39．5％；불잉환자조AsAb양성솔25．5％（51/200）여건강대조조 AsAb 양성솔1％（1/100）비교，차이유통계학의의（P＝0．000）；불잉환자조ACA양성솔위24％（48/200）여건강대조조ACA 양성솔1％（1/100）비교，차이유통계학의의（P＝0．000）。AsAb-IgG、AsAb-IgM、AsAb-IgA화ACA-IgM、ACA-IgG양성솔여대조조비교차이균유통계학의의（P＜0．05）；ACA-IgA 양성솔여대조조비교차이무통계학의의（P＞0．05）；AsAb-IgM화ACA-IgG재불잉불육조내여기타류형항체양성솔비교，차이균유통계학의의（P＜0．05）。AsAb、ACA양성솔재불동년령단간여대조조비교，3조차이균유통계학의의（P＜0．05）。결론 AsAb、ACA 시인기불잉불육적중요적면역인소，차AsAb-IgM화ACA-IgG여녀성불잉불육적관계경가긴밀。저사항체대불동년령인군적불잉불육환자균유영향，인차AsAb、ACA 적검측대면역성인소인기불잉불육환자적진단화치료구유림상개치。
Objective To investigate the detective value of anti-sperm antibody (AsAb)and anti-cardiolipin antibody (ACA)in patients with female infertility.Methods 200 female patients diagnosed with infertility and 100 healthy adults were selected in ma-ternal and child health hospital in Hunan province from January 2012 to December 2012.The enzyme-linked immunosorbent assay (ELISA)was used to detect serum AsAb and ACA of the two groups and the antibody-positive rates were compared.ResultsThere were 79 positive cases of these two reproductive immune antibody of serum and the positive rate was 39.5%.The positive rates of AsAb were 25.5% (51/200)in infertility patients group and 1% (1/100)in the control group,and the difference was sig-nificant (P= 0.000).The positive rates of ACA were 14% (28/200)in infertility patients group and 1% (1/100)in the control group,and the difference was significant (P= 0.000).Compared with the control group,the positive rate of AsAb-IgG,AsAb-IgM,AsAb-IgA,ACA-IgM and ACA-IgG had significant difference (P<0.05).Compared with the control group,the positive rate of ACA-IgA was not significantly different (P>0.05).And the positive rate of AsAb-IgM and ACA-IgG in the infertility group compared with other types of antibody,the differences were statistically significant (P<0.05).And compared with the control group,the positive rate of AsAb and ACA in different age groups was significantly different (P<0.05).Conclusion AsAb and ACA were important immune factors caused infertility.AsAb-IgM and ACA-IgG were more closely related to female infertility. These antibodies affect the patients with infertility at different age groups.Detection of these antibodies is of important guiding sig-nificance for diagnosis and treatment of immune infertility patients.