CAJ | 학술논문

目的：探讨不同羊水细胞处理方法对ABO 血型基因鉴定的影响。方法选取孕16～25周年龄21～38岁的孕妇53例，每例采集羊水标本2份，一份进行羊水细胞培养2～5 d，收集培养的羊水细胞；另一份直接离心分离羊水细胞，然后提取羊水细胞DNA，采用序列特异引物引导的PCR反应（PCR-SSP）基因检测技术鉴定羊水细胞的ABO基因型，待婴儿出生后，抽取其外周血或脐带血进行微柱凝胶ABO血型检测。结果羊水细胞培养法有5例未检出，检出率为90．5％（48/53）；直接法中有9例未检出，其中有3例结果无法识别，结果检出率为83．0％（44/53）；24例新生儿的外周血或脐带血的微柱凝胶ABO血型检测结果显示有1例无结果，与基因型的结果比对完全一致。结论羊水细胞可直接进行PCR-SSP检测ABO血型，细胞培养法优于直接分析法。
목적：탐토불동양수세포처리방법대ABO 혈형기인감정적영향。방법선취잉16～25주년령21～38세적잉부53례，매례채집양수표본2빈，일빈진행양수세포배양2～5 d，수집배양적양수세포；령일빈직접리심분리양수세포，연후제취양수세포DNA，채용서렬특이인물인도적PCR반응（PCR-SSP）기인검측기술감정양수세포적ABO기인형，대영인출생후，추취기외주혈혹제대혈진행미주응효ABO혈형검측。결과양수세포배양법유5례미검출，검출솔위90．5％（48/53）；직접법중유9례미검출，기중유3례결과무법식별，결과검출솔위83．0％（44/53）；24례신생인적외주혈혹제대혈적미주응효ABO혈형검측결과현시유1례무결과，여기인형적결과비대완전일치。결론양수세포가직접진행PCR-SSP검측ABO혈형，세포배양법우우직접분석법。
Objective To explore the effect of different treatment methods for amniotic fluid cells on ABO gene identification by polymerase chain reaction with sequence-specific primers (PCR-SSP).Methods Fifty three pregnant women with gestational age from 16 to 25 weeks were selected.To extract double amniotic fluid specimens,one was for in vitro cell culture and the other was for separating aminotic cell using centrifuge.ABO genotypes were detected by PCR-SSP after DNA was extracted from the amniot-ic cells.After fetus gave birth,peripheral blood was collected from these newborns and was analyzed by microcolumn gel agglutina-tion assay.Results Among the 53 specimens from cell culture method,ABO genotype of 5 cases were not detected.The detection rate was 90.5%(48/53).There were nine cases that were not detected in direct centrifuge method group and the detection rate was 83.0%(44/53).Among them,results of 3 cases could not identify.Among the twenty-four neonatal peripheral blood or cord blood cases detected by microcolumn gel agglutination assay,there was one case that not detected.The results were same with that of PCR-SSP.Conclusion ABO genotype can be analyzed in amniotic fluid cells by PCR-SSP,and the cell culture method is advanced than direct centrifuge method.