植物保护
植物保護
식물보호
PLANT PROTECTION
2014年
5期
106-110
,共5页
程保平%彭埃天%宋晓兵%凌金锋%陈霞
程保平%彭埃天%宋曉兵%凌金鋒%陳霞
정보평%팽애천%송효병%릉금봉%진하
柑橘黄龙病%常规 PCR%巢式 PCR%实时荧光定量 PCR%比较研究
柑橘黃龍病%常規 PCR%巢式 PCR%實時熒光定量 PCR%比較研究
감귤황룡병%상규 PCR%소식 PCR%실시형광정량 PCR%비교연구
Candidatus Liberibacter asiaticus%conventional PCR%nested-PCR%real time quantitative PCR%comparative research
为了比较常规 PCR、巢式 PCR 和实时荧光定量 PCR 方法在大田检测中对柑橘黄龙病（Huanglongbing， HLB）的检测效果，首先比较了3种检测方法对柑橘黄龙病菌检测的灵敏度，结果发现：3种检测方法的灵敏度依次为常规 PCR＜巢式 PCR＜实时荧光定量 PCR。运用3种检测方法对广东5个柑橘品种上的189个黄龙病疑似病样进行检测，结果发现：黄龙病检出率依次为常规 PCR＜巢式 PCR＜实时荧光定量 PCR。研究表明：常规 PCR 适合以较低成本大规模检测黄龙病；实时荧光定量 PCR 具有最大的检测灵敏度；巢式 PCR 检测技术同时具有前两者的一些优点，但操作较复杂，适合技术熟练的研究者使用。
為瞭比較常規 PCR、巢式 PCR 和實時熒光定量 PCR 方法在大田檢測中對柑橘黃龍病（Huanglongbing， HLB）的檢測效果，首先比較瞭3種檢測方法對柑橘黃龍病菌檢測的靈敏度，結果髮現：3種檢測方法的靈敏度依次為常規 PCR＜巢式 PCR＜實時熒光定量 PCR。運用3種檢測方法對廣東5箇柑橘品種上的189箇黃龍病疑似病樣進行檢測，結果髮現：黃龍病檢齣率依次為常規 PCR＜巢式 PCR＜實時熒光定量 PCR。研究錶明：常規 PCR 適閤以較低成本大規模檢測黃龍病；實時熒光定量 PCR 具有最大的檢測靈敏度；巢式 PCR 檢測技術同時具有前兩者的一些優點，但操作較複雜，適閤技術熟練的研究者使用。
위료비교상규 PCR、소식 PCR 화실시형광정량 PCR 방법재대전검측중대감귤황룡병（Huanglongbing， HLB）적검측효과，수선비교료3충검측방법대감귤황룡병균검측적령민도，결과발현：3충검측방법적령민도의차위상규 PCR＜소식 PCR＜실시형광정량 PCR。운용3충검측방법대엄동5개감귤품충상적189개황룡병의사병양진행검측，결과발현：황룡병검출솔의차위상규 PCR＜소식 PCR＜실시형광정량 PCR。연구표명：상규 PCR 괄합이교저성본대규모검측황룡병；실시형광정량 PCR 구유최대적검측령민도；소식 PCR 검측기술동시구유전량자적일사우점，단조작교복잡，괄합기술숙련적연구자사용。
Detection sensitivity and detection rate of conventional PCR,nested PCR and real-time quantitative PCR were compared for detection of citrus huanglongbing.The results showed that the detection sensitivity was ranked from high to low as real-time quantitative PCR>nested PCR>conventional PCR.Then total 189 suspec-ted citrus samples from five different cultivars with symptom of HLB in orchard of Guangdong were amplified and confirmed by the three methods.The results indicated that the detection rate was ranked from high to low as real-time quantitative PCR>nested PCR>conventional PCR.This research indicated that conventional PCR,du to its low cost,could be used in large scale diagnosis of HLB disease,real-time quantitative PCR had the highest detec-tion sensitivity,and nested-PCR had the advantages of the other two,but its experimental operation was more complex.
