CAJ | 학술논문

目的：研究绞股蓝皂甙对人衰老皮肤成纤维细胞增殖的影响及机制。方法原代培养并建立人衰老皮肤成纤维细胞系，0、5、10、15μg/mL绞股蓝皂甙处理细胞，24、72、96 h后MTT检测细胞增殖；72h后流式细胞仪检测细胞周期。结果10、15μg/mL绞股蓝皂甙可以促进人衰老成纤维细胞增殖，使 S期细胞数增多且呈浓度依赖。结论绞股蓝皂甙可以使S期细胞数增多且成纤维细胞增殖能力增强，具有延缓细胞衰老的作用。
목적：연구교고람조대대인쇠로피부성섬유세포증식적영향급궤제。방법원대배양병건립인쇠로피부성섬유세포계，0、5、10、15μg/mL교고람조대처리세포，24、72、96 h후MTT검측세포증식；72h후류식세포의검측세포주기。결과10、15μg/mL교고람조대가이촉진인쇠로성섬유세포증식，사 S기세포수증다차정농도의뢰。결론교고람조대가이사S기세포수증다차성섬유세포증식능력증강，구유연완세포쇠로적작용。
Objective To study the ef ect of Gypenosides on the proliferation of aged fibroblasts. Methods Fibroblasts were cultured and fed by Gypenosides with 0μg/ml,5μg/ml,10μg/ml and 15μg/ml for 24h,72h and 96h. cellproliferation and cellcycle were tested by MTT method and flow cytometer. Results Gypenosides could promote aged fibroblasts proliferation. At the same time,increased the number of cells in S stage. Conclusions Gypenosides can promote the proliferation of the aged fibroblasts and therefore could delay the cells ageing.