植物保护
植物保護
식물보호
PLANT PROTECTION
2014年
5期
55-61
,共7页
PS21 复合菌剂%氧乐果%幼苗生长%小麦%抗氧化酶系
PS21 複閤菌劑%氧樂果%幼苗生長%小麥%抗氧化酶繫
PS21 복합균제%양악과%유묘생장%소맥%항양화매계
compound bacteria PS21%omethoate%seedling growth%wheat%antioxidant enzyme system
采用溶液培养法研究了不同浓度的复合菌剂 PS21与氧乐果协同处理对小麦幼苗生长及抗氧化酶的影响，初步探讨复合菌剂 PS21缓解氧乐果对小麦生长和抗氧化酶的抑制效应。研究结果表明，106～108 cfu/mL 浓度的复合菌剂 PS21对氧乐果的毒害具有缓解作用，缓解作用与复合菌剂 PS21浓度呈正相关性，菌液浓度为108 cfu/mL时，缓解作用明显，与氧乐果单独处理相比，小麦株高、茎粗、干重、湿重、叶绿素含量显著增加；在抗氧化酶系统中随复合菌剂 PS21浓度的升高，幼苗叶片超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性均有不同程度的提高，丙二醛（MDA）含量下降。同浓度复合菌剂 PS21的不同施用方法对氧乐果毒害的缓解作用存在差异，灌根法对氧乐果毒害小麦具有较强的缓解作用。
採用溶液培養法研究瞭不同濃度的複閤菌劑 PS21與氧樂果協同處理對小麥幼苗生長及抗氧化酶的影響，初步探討複閤菌劑 PS21緩解氧樂果對小麥生長和抗氧化酶的抑製效應。研究結果錶明，106～108 cfu/mL 濃度的複閤菌劑 PS21對氧樂果的毒害具有緩解作用，緩解作用與複閤菌劑 PS21濃度呈正相關性，菌液濃度為108 cfu/mL時，緩解作用明顯，與氧樂果單獨處理相比，小麥株高、莖粗、榦重、濕重、葉綠素含量顯著增加；在抗氧化酶繫統中隨複閤菌劑 PS21濃度的升高，幼苗葉片超氧化物歧化酶（SOD）、過氧化氫酶（CAT）和過氧化物酶（POD）活性均有不同程度的提高，丙二醛（MDA）含量下降。同濃度複閤菌劑 PS21的不同施用方法對氧樂果毒害的緩解作用存在差異，灌根法對氧樂果毒害小麥具有較彊的緩解作用。
채용용액배양법연구료불동농도적복합균제 PS21여양악과협동처리대소맥유묘생장급항양화매적영향，초보탐토복합균제 PS21완해양악과대소맥생장화항양화매적억제효응。연구결과표명，106～108 cfu/mL 농도적복합균제 PS21대양악과적독해구유완해작용，완해작용여복합균제 PS21농도정정상관성，균액농도위108 cfu/mL시，완해작용명현，여양악과단독처리상비，소맥주고、경조、간중、습중、협록소함량현저증가；재항양화매계통중수복합균제 PS21농도적승고，유묘협편초양화물기화매（SOD）、과양화경매（CAT）화과양화물매（POD）활성균유불동정도적제고，병이철（MDA）함량하강。동농도복합균제 PS21적불동시용방법대양악과독해적완해작용존재차이，관근법대양악과독해소맥구유교강적완해작용。
The seedlings of wheat were cultured in liquid media containing omethoate,or the combination of omethoate and compound bacteria PS21.The seedling growth and antioxidant enzyme activities were examined for each medium,in order to explore the influence of compound bacteria PS21 on seedling growth and antioxidant en-zymes activities in wheat under the stress of omethoate.The results showed that the different concentrations of compound bacteria could alleviate the omethoate toxicity in wheat plants to various degrees,and there was a posi-tive correlation between alleviative effect and compound bacteria PS21 concentration.Under 108 cfu/mL PS21 treatment,the omethoate stress suffered by wheat seedlings could be obviously alleviated.Compared to sole omethoate treatment,compound bacteria PS21 could remarkably increase plant height,stem diameter,dry weight,wet weight,and the contents of chlorophyll.With increasing concentration of compound bacteria PS21, antioxidant enzyme activities of superoxide dismutase (SOD),catalase (CAT)and peroxidase (POD)increased to various degrees,whereas malondialdehyde (MDA)contents of seedling leaves decreased.The relieving effects were different among two application methods of compound bacteria PS21.Root-irrigating method significantly alleviated omethoate toxicity in wheat plants.
