CAJ | 학술논문

以我国高寒地区分离、筛选出的高致病力昆虫病原线虫嗜菌异小杆线虫（Heterorhabditis bacteriophora-HBN）为研究对象，对其冷冻储存条件进行优化。结果显示，介质中不同含水量对线虫 HBN 的冷冻存活率有着显著的影响；其中含水量为50％的蛭石中线虫回收率最高，为87．4％；冷冻储存后的线虫经4℃24 h＋室温1 h 解冻利于线虫冷冻休眠后复苏；线虫经阶段性降温较直接降温至-4℃的冷冻存活率提高31．6百分点；储存介质蛭石中采用灭菌自来水较适于 HBN 线虫在-4℃冷冻存活；7 d 内冷冻储存增强了 HBN 线虫对寄主昆虫的侵入能力。
이아국고한지구분리、사선출적고치병력곤충병원선충기균이소간선충（Heterorhabditis bacteriophora-HBN）위연구대상，대기냉동저존조건진행우화。결과현시，개질중불동함수량대선충 HBN 적냉동존활솔유착현저적영향；기중함수량위50％적질석중선충회수솔최고，위87．4％；냉동저존후적선충경4℃24 h＋실온1 h 해동리우선충냉동휴면후복소；선충경계단성강온교직접강온지-4℃적냉동존활솔제고31．6백분점；저존개질질석중채용멸균자래수교괄우 HBN 선충재-4℃냉동존활；7 d 내냉동저존증강료 HBN 선충대기주곤충적침입능력。
One high-virulence strain of EPNs,Heterorhabditis bacteriophora-HBN (HBN)with cold tolerance iso-lated from Harbin City was used in the laboratory in this study.Storage substance species and moisture,cooling rate,thawing ways and osmotic pressure of solution in the substances were optimized.The results showed that freezing survival of HBN were significantly affected by the substances with different water contents.Recycling co-efficient of H .bacteriophora-HBN in the vermiculite with water content of 50% was the highest (87.4%).The way of freezing HBN at room temperature for 1h following 4 ℃ for 24h was in favor of thawing of freezing dor-mancy.Freezing survival rate of HBN by phasic cooling pattern was increased by 31.6 percent than by direct cool-ing to -4 ℃.Sterilized artificial tap water in vermiculite was conducive to anhydrobiosis of HBN.The ability of HBN infecting Galleria mellonella was enhanced in the short-term freezing storage in seven days.