CAJ | 학술논문

目的：研究超声及添加细胞分泌促进剂、培养液增稠剂对喜树细胞悬浮培养的影响。方法采用不同超声时间、不同浓度的 DMSO 和CMC-Na作用于悬浮生长的喜树细胞，观察喜树细胞的生长情况。结果10s 超声波作用及0.5％ DMSO 可显著增加细胞生物量，添加1％CMC-Na培养的喜树细胞几乎呈单细胞状态。结论施加适当的超声、DMSO和CMC-Na处理可显著影响悬浮培养喜树细胞生物量和生长状态。
목적：연구초성급첨가세포분비촉진제、배양액증주제대희수세포현부배양적영향。방법채용불동초성시간、불동농도적 DMSO 화CMC-Na작용우현부생장적희수세포，관찰희수세포적생장정황。결과10s 초성파작용급0.5％ DMSO 가현저증가세포생물량，첨가1％CMC-Na배양적희수세포궤호정단세포상태。결론시가괄당적초성、DMSO화CMC-Na처리가현저영향현부배양희수세포생물량화생장상태。
OBJECTIVE To study the infulences of ultrasonic wave ,permeabilization and viscous additive-sup-plemented medium on cell cultures of Camptotheca acuminata.METHODS The cell cultures were treated with dif-ferent durations of ultrasonic wave and different concentrations of DMSO and CMC-Na and the growth rates of cells were investigated.RESULTS Ten seconds of ultrasonic wave and 0.5%DMSO could significantly improve the cell biomass and the cells grew seperately when the medium was added with 1%CMC-Na.CONCLUSION Appropriate ultrasonic wave ,DMSO and CMC-Na can significantly affect the biomass and cell status of Camptotheca acuminata.