CAJ | 학술논문

目的：探讨一管式多引物焦磷酸测序技术在人类乳头瘤病毒（HPV ）分型检测中的临床应用价值。方法设计针对7个不同 HPV亚型的高度特异性测序引物，采用一管式多引物测序法，通过半巢式聚合酶链反应（PCR）扩增获得 HPV基因片段，并进行焦磷酸测序分析。结果通过半巢式PCR扩增和一管式多引物焦磷酸测序，成功区分了7种不同的 HPV亚型，且测序结果与PCR-反向点杂交法检测结果的符合率是100％。结论一管式多引物焦磷酸测序技术是一种简便、高效、高通量、高灵敏度、高准确度的HPV分型检测方法，值得推广应用。
목적：탐토일관식다인물초린산측서기술재인류유두류병독（HPV ）분형검측중적림상응용개치。방법설계침대7개불동 HPV아형적고도특이성측서인물，채용일관식다인물측서법，통과반소식취합매련반응（PCR）확증획득 HPV기인편단，병진행초린산측서분석。결과통과반소식PCR확증화일관식다인물초린산측서，성공구분료7충불동적 HPV아형，차측서결과여PCR-반향점잡교법검측결과적부합솔시100％。결론일관식다인물초린산측서기술시일충간편、고효、고통량、고령민도、고준학도적HPV분형검측방법，치득추엄응용。
Objective To investigate the significance of pyrosequencing in the genotyping of human papilloma-viruses (HPV) .Methods Seven highly specific sequencing primers for HPV subtypes were designed and used to am-plify HPV DNA fragments by semi-nested polymerase chain reaction (PCR) .Then ,the PCR products were se-quenced by pyrosequencing .Results Seven different HPV subtypes were successfully identified by semi-nested PCR and pyrosequencing with seven sequencing primers mixed in one well .Conclusion Pyrosequencing using multi-prim-ers mixed in one well could be an efficient way to detect different HPV subtypes .