CAJ | 학술논문

采用室内生物测定和生化分析方法，以采自甘肃兰州国家级森林公园兴隆山的二斑叶螨为敏感品系（SS），研究二斑叶螨对阿维菌素的抗药性及抗性生化机理。结果表明：在室内用阿维菌素强化筛选24代，获得了二斑叶螨抗阿维菌素品系（Ab-R24），抗性指数（resistance index，RI）为321．5。对 SS 和 Ab-R24解毒酶活性的分析表明， Ab-R24品系体内羧酸酯酶（CarE）、乙酰胆碱酯酶（AchE）、酸性磷酸酯酶（ACP）、碱性磷酸酯酶（ALP）、谷胱甘肽S-转移酶（GSTs）和多功能氧化酶（MFO）活性分别是 SS 品系的1．43、1．18、1．56、1．48、1．55倍和4．02倍，差异达到显著水平（P＜0．05），其中 MFO 的活性上升最为显著。对 SS 和 Ab-R24解毒酶动力学常数的分析表明，Ab-R24品系体内 AchE、GSTs 和 MFO 的 Km 分别是 SS 品系的1．14、2．31倍和2．58倍；Vmax 分别是 SS 品系的1．19、2．34倍和1．76倍，差异均达到显著水平（P＜0．05）。说明二斑叶螨对阿维菌素抗性增高与 MFO 活性快速升高有关，AchE 和GSTs 也参与阿维菌素抗性的形成。
채용실내생물측정화생화분석방법，이채자감숙란주국가급삼림공완흥륭산적이반협만위민감품계（SS），연구이반협만대아유균소적항약성급항성생화궤리。결과표명：재실내용아유균소강화사선24대，획득료이반협만항아유균소품계（Ab-R24），항성지수（resistance index，RI）위321．5。대 SS 화 Ab-R24해독매활성적분석표명， Ab-R24품계체내최산지매（CarE）、을선담감지매（AchE）、산성린산지매（ACP）、감성린산지매（ALP）、곡광감태S-전이매（GSTs）화다공능양화매（MFO）활성분별시 SS 품계적1．43、1．18、1．56、1．48、1．55배화4．02배，차이체도현저수평（P＜0．05），기중 MFO 적활성상승최위현저。대 SS 화 Ab-R24해독매동역학상수적분석표명，Ab-R24품계체내 AchE、GSTs 화 MFO 적 Km 분별시 SS 품계적1．14、2．31배화2．58배；Vmax 분별시 SS 품계적1．19、2．34배화1．76배，차이균체도현저수평（P＜0．05）。설명이반협만대아유균소항성증고여 MFO 활성쾌속승고유관，AchE 화GSTs 야삼여아유균소항성적형성。
In order to study the insecticide resistance and resistance biochemistry mechanism of Tetranychus urti-cae to abamectin,we used bioassay and biochemical analysis methods.In addition,T .urticae from Xinglong Mountain in Gansu Lanzhou National Forest Park was used as a susceptible strain (SS).The results indicated that T .urticae abamectin-resistance 24 (Ab-R24 )was obtained after indoor screening for 24 generations with abamec-tin,whose resistance index (RI)was 321.5.The analysis of SS and Ab-R24 detoxification enzyme activity showed that the activities of carboxylesterases (CarE),acetylcholinesterase (AchE),acid phosphatase (ACP),alkaline phosphatase (ALP),glutathione S-transferase (GSTs)and mixed function oxidase (MFO)of Ab-R24 strain were in vivo 1.43,1.18,1.56,1.48,1.55 and 4.02 times of the corresponding activity of SS,respectively,which reached the significance level (P <0.05).The analysis of SS and Ab-R24 detoxifying enzyme kinetic constants showed that the Km values of AchE/GSTs and MFO of Ab-R24 strain were in vivo 1.14,2.31 and 2.58 times of the cor-responding value of SS,respectively;at the same time,the Vmax values were 1.19,2.34 and 1.76 times of the corre-sponding value of SS,respectively,which reached the significance level (P<0.05).In conclusion,the activity of MFO rose most obviously,indicating that the rise of T.urticae’s abamectin resistance was closely related with the rapid rise of MFO’s activity.There is no doubt that AchE and GSTs are both involved in the formation of abamectin resistance.