植物保护
植物保護
식물보호
PLANT PROTECTION
2014年
5期
40-43
,共4页
陈利军%智亚楠%王国君%史洪中
陳利軍%智亞楠%王國君%史洪中
진리군%지아남%왕국군%사홍중
土荆芥%挥发油%GC-MS 分析%抑菌作用%不同部位
土荊芥%揮髮油%GC-MS 分析%抑菌作用%不同部位
토형개%휘발유%GC-MS 분석%억균작용%불동부위
Chenopodium ambrosioides%essential oil%GC-MS analysis%antifungal activity%different parts
采用水蒸气蒸馏法分别提取采自河南信阳的土荆芥花序和叶的挥发油，采用 GC-MS 进行组分分析，并测定挥发油的抑菌作用。结果表明：土荆芥花序和叶的挥发油的含油率分别为4．02％和1．64％；GC-MS 从挥发油中分别分离到10个和9个组分，均鉴定出7个组分，占挥发油总量的98．431％和99．103％；挥发油的主要组分均为驱蛔素、2-蒈烯和α-松油烯，但相对含量差异较大；叶和花序的挥发油对小麦赤霉病菌具有强烈的熏蒸抑制作用，EC50分别为1．46μL/皿和1．80μL/皿。
採用水蒸氣蒸餾法分彆提取採自河南信暘的土荊芥花序和葉的揮髮油，採用 GC-MS 進行組分分析，併測定揮髮油的抑菌作用。結果錶明：土荊芥花序和葉的揮髮油的含油率分彆為4．02％和1．64％；GC-MS 從揮髮油中分彆分離到10箇和9箇組分，均鑒定齣7箇組分，佔揮髮油總量的98．431％和99．103％；揮髮油的主要組分均為驅蛔素、2-蒈烯和α-鬆油烯，但相對含量差異較大；葉和花序的揮髮油對小麥赤黴病菌具有彊烈的熏蒸抑製作用，EC50分彆為1．46μL/皿和1．80μL/皿。
채용수증기증류법분별제취채자하남신양적토형개화서화협적휘발유，채용 GC-MS 진행조분분석，병측정휘발유적억균작용。결과표명：토형개화서화협적휘발유적함유솔분별위4．02％화1．64％；GC-MS 종휘발유중분별분리도10개화9개조분，균감정출7개조분，점휘발유총량적98．431％화99．103％；휘발유적주요조분균위구회소、2-개희화α-송유희，단상대함량차이교대；협화화서적휘발유대소맥적매병균구유강렬적훈증억제작용，EC50분별위1．46μL/명화1．80μL/명。
Essential oils from the flowers and leaves of Chenopodium ambrosioides L.in Xinyang City of Henan Province were extracted by steam distillation,and their chemical constituents were analyzed by GC-MS.The anti-fungal activity of essential oils was also studied.The results showed that the yields of essential oils from the flowers and leaves were 4.02% and 1.64%,respectively.Ten and 9 constituents were isolated,and 7 constituents were identified,accounting for 98.431% and 99.103% of the total essential oils from the flowers and leaves by GC-MS,respectively.The main chemical constituents of all essential oils were ascaridole,2-carene and α-terpinene, but the relative contents varied.The essential oils from leaves and flowers showed strong fumigation antifungal ac-tivity to Fusarium graminearum ,with an EC50 value of 1.46 μL/dish and 1.80 μL/dish,respectively.
